Rabbit ATG101 Polyclonal Antibody | anti-ATG101 antibody

ATG101 Antibody

Cat. Num. AAA9411535

• Gene Names: ATG101; C12orf44
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: ATG101; Polyclonal Antibody; ATG101 Antibody; Autophagy-related protein 101; ATG13-interacting protein; anti-ATG101 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 218

• Applicable Applications for anti-ATG101 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 16 amino acid peptide near the center of human ATG101.
• Target Name: ATG101
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 ug/mL.)

Related Product Information for anti-ATG101 antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.

Product Categories/Family for anti-ATG101 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 149158714
• NCBI GeneID: 60673
• NCBI Accession #: NP_001092143
• NCBI GenBank Nucleotide #: NM_001098673.1
• Molecular Weight: 25,003 Da
• NCBI Official Full Name: autophagy-related protein 101
• NCBI Official Synonym Full Names: autophagy related 101
• NCBI Official Symbol: ATG101
• NCBI Official Synonym Symbols: C12orf44
• NCBI Protein Information: autophagy-related protein 101
• UniProt Protein Name: Autophagy-related protein 101
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG101
• UniProt Synonym Gene Names: C12orf44
• UniProt Entry Name: ATGA1_HUMAN

Uniprot Description

ATG101: an protein required for autophagosome formation. Interacts with ATG13. Associates with a complex composed of ATG13, ULK1 and RB1CC1. Under starvation conditions it localizes to phagophores, which are preautophagosomal structures. Stabilizes the expression of Atg13 in the cell. Belongs to the ATG101 family.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 12q13.13

Molecular Function: identical protein binding; protein binding; protein complex binding

Biological Process: autophagic vacuole formation