Rabbit ATG9A Polyclonal Antibody | anti-ATG9A antibody

ATG9A Antibody

Cat. Num. AAA150586

• Gene Names: ATG9A; mATG9; APG9L1; MGD3208
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: ATG9A Antibody is affinity chromatography purified via peptide column.
• Synonyms: ATG9A; Polyclonal Antibody; ATG9A Antibody; mATG9; APG9L1; MGD3208; Autophagy-related protein 9A; APG9-like 1; ATG9 autophagy related 9 homolog A (S. cerevisiae); anti-ATG9A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ATG9A Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 839

• Applicable Applications for anti-ATG9A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: ATG9A antibody can be used for detection of ATG9A by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A.
• Buffer: ATG9A Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ATG9A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ATG9A in mouse heart tissue lysate with ATG9A antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ATG9A in human heart tissue with ATG9A antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ATG9A in human heart tissue with ATG9A antibody at 20 μg/mL.)

Related Product Information for anti-ATG9A antibody

ATG9A Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.

NCBI and Uniprot Product Information

• NCBI GI #: 116089287
• NCBI GeneID: 79065
• NCBI Accession #: NP_076990
• NCBI GenBank Nucleotide #: NM_024085.4
• UniProt Accession #: Q7Z3C6; Q3ZAQ6; Q6P0N7; Q7Z317; Q7Z320; Q8NDK6; Q8WU65; Q9BVL5; Q9H6L1; Q9HAG7
• Molecular Weight: 60,694 Da
• NCBI Official Full Name: autophagy-related protein 9A
• NCBI Official Synonym Full Names: autophagy related 9A
• NCBI Official Symbol: ATG9A
• NCBI Official Synonym Symbols: mATG9; APG9L1; MGD3208
• NCBI Protein Information: autophagy-related protein 9A; APG9-like 1; APG9 autophagy 9-like 1; autophagy 9-like 1 protein; ATG9 autophagy related 9 homolog A
• UniProt Protein Name: Autophagy-related protein 9A
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG9A
• UniProt Synonym Gene Names: APG9L1
• UniProt Entry Name: ATG9A_HUMAN

Uniprot Description

ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: endoplasmic reticulum membrane; recycling endosome; membrane; late endosome membrane; late endosome; integral to membrane; autophagic vacuole; cytoplasmic vesicle; trans-Golgi network; endosome

Molecular Function: protein binding

Biological Process: protein transport; protein localization in Golgi apparatus; mitochondrion degradation; innate immune response; autophagic vacuole formation