Human Vitamin DBP ELISA Kit | DBP elisa kit

Human Vitamin DBP ELISA Kit

Cat. Num. AAA1753889

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; DBP/GC
• Reactivity: Human
• Synonyms: Vitamin DBP; Human Vitamin DBP ELISA Kit; Vitamin D-binding protein; DBP; VDB; Gc protein-derived macrophage activating factor ; GC; vitamin D binding protein; DBP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Vitamin DBP

• Samples: cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk
• Detection Range: 1.56ng/ml-100ng/ml
• Sensitivity: <150pg/ml
• Capture/Detection Antibodies: Capture antibody- a monoclonal antibody from mouse|Detection antibody- a biotinylated polyclonal antibody from goat
• Sample Data: Concentration(ng/ml)|0|1.56|3.12|6.25|12.5|25|50|100; O.D.|0.008|0.103|0.175|0.316|0.584|0.992|1.600|2.167
• Intra-Assay Precision: Sample|1|2|3|; n|16|16|16|; Mean(pg/ml)|3726|12058|49927|; Standard deviation|182.57|940.52|2346.56|; CV(%)|4.9%|7.8%|4.7%|
• Inter-Assay Precision: Sample|1|2|3|; n|24|24|24; Mean(pg/ml)|3646|11478|49590; Standard deviation|236.99|1033.02|2578.68; CV(%)|6.5%|9%|5.2%
• Reproducibility: To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.; Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%); Sample 1: 3726|3810|3849|3865|3812|53.79|1.4%; Sample 2: 12058|12135|11622|10357|11543|712.13|6.1%; Sample 3: 49927|48196|52754|50855|50433|1645.09|3.2%; *number of samples for each test n=16.
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L17-L474
• Anticoagulant: heparin or EDTA
• Principle: Human Vitamin DBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Vitamin DBP has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: L17-L474) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Vitamin DBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Vitamin DBP amount of sample captured in plate.
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human Vitamin DBP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" for details. It is recommended that each human Vitamin DBP standard solution and each sample be measured in duplicate.
• TMB Incubation Time: 15-20
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Vitamin DBP ELISA Kit standard curve)

Related Product Information for DBP elisa kit

Vitamin D-binding protein, also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene. The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.

Product Categories/Family for DBP elisa kit

ELISA kits

NCBI and Uniprot Product Information

• NCBI GI #: 324021745
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191236.1
• NCBI GenBank Nucleotide #: NM_001204307.1
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 52,964 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 3
• NCBI Official Synonym Full Names: group-specific component (vitamin D binding protein)
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; DBP/GC
• NCBI Protein Information: vitamin D-binding protein; VDB; gc-globulin; vitamin D-binding alpha-globulin
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Remorin
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP; VDB
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1