Rabbit GLK Polyclonal Antibody | anti-GLK antibody

GLK (G4) Polyclonal Antibody

Cat. Num. AAA3003011

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GLK; Polyclonal Antibody; GLK (G4) Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase kinase 3; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; Germinal center kinase related protein kinase; MEKKK 3; MAP4K3; anti-GLK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GLK (G4) polyclonal antibody detects endogenous levels of GLK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 873

• Applicable Applications for anti-GLK antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to the N-terminal of Human GLK.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GLK (G4) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-GLK antibody

Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 394025669
• NCBI GeneID: 8491
• NCBI Accession #: NP_001257354.1
• NCBI GenBank Nucleotide #: NP_001257354.1
• UniProt Accession #: Q8IVH8
• Molecular Weight: ~ 65kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Glucokinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV