Goat ASNA1 Polyclonal Antibody | anti-ASNA1 antibody

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40)

Cat. Num. AAA648748

• Gene Names: ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I; hASNA-I
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ASNA1; Polyclonal Antibody; ASNA1 (ATPase ASNA1; Arsenical Pump-driving ATPase; Arsenite-stimulated ATPase; Transmembrane Domain Recognition Complex 40kD ATPase Subunit; hARSA-I; hASNA-I; ARSA; TRC40); Anti -ASNA1 (ATPase ASNA1; anti-ASNA1 antibody

• Host: Goat
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ASNA1. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-ASNA1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:2000; Western Blot: 0.3-1ug/ml, observed in human, mouse and rat kidney lysates on ~38kD bands
• Immunogen: Synthetic peptide corresponding to C-PHEVRGADKVNT, from ASNA1, at the internal region of the protein (NP_004308.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ASNA1 antibody

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling.

Product Categories/Family for anti-ASNA1 antibody

Antibodies; Abs to Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 50428938
• NCBI GeneID: 439
• NCBI Accession #: NP_004308.2
• NCBI GenBank Nucleotide #: NM_004317.2
• UniProt Accession #: O43681; Q53FC6; Q92849; A6NHP8; A8K740
• Molecular Weight: 38,793 Da
• NCBI Official Full Name: ATPase ASNA1
• NCBI Official Synonym Full Names: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• NCBI Official Symbol: ASNA1
• NCBI Official Synonym Symbols: GET3; ARSA1; TRC40; ARSA-I; ASNA-I; hASNA-I
• NCBI Protein Information: ATPase ASNA1; arsenite-stimulated ATPase; arsenical pump-driving ATPase; golgi to ER traffic 3 homolog; transmembrane domain recognition complex, 40kDa; transmembrane domain recognition complex 40 kDa ATPase subunit
• UniProt Protein Name: ATPase ASNA1
• Protein Family: ATPase
• UniProt Gene Name: ASNA1
• UniProt Synonym Gene Names: ARSA; TRC40
• UniProt Entry Name: ASNA_HUMAN

NCBI Description

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]

Uniprot Description

Function: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting

By similarity. May be involved in insulin signaling. Ref.12 Ref.13

Subunit structure: Homodimer

By similarity. Component of a transmembrane domain recognition complex (TRC)

By similarity. Interacts with SERP1 and SEC61B

By similarity. Interacts with WRB. Ref.12 Ref.13 Ref.16

Subcellular location: Cytoplasm. Endoplasmic reticulum. Nucleus › nucleolus Ref.10 Ref.12 Ref.16.

Tissue specificity: Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle. Ref.11

Sequence similarities: Belongs to the arsA ATPase family.

Biophysicochemical propertiesKinetic parameters:KM=0.22 mM for ATP Ref.9Vmax=16.6 nmol/min/mg enzyme for ATP

Sequence caution: The sequence AAC50731.1 differs from that shown. Reason: Frameshift at position 19.