Testing Data
(Translected human embryonic kidney 293 cells, overexpressing chicken Dscam or mouse Dscam, fixed with 4% paralormaldehyde/PBS. Cells are labeled with anti-Dscam (DS2-176) antibody)
Mouse Down Syndrome Cell Adhesion Molecule (DSCAM) Monoclonal Antibody | anti-DSCAM antibody
Down Syndrome Cell Adhesion Molecule (DSCAM) Antibody (DS2-176)
Reactivity
Chicken, Mouse
Applications
Immunohistochemistry, Western Blot
Synonyms
Down Syndrome Cell Adhesion Molecule (DSCAM); Monoclonal Antibody; Down Syndrome Cell Adhesion Molecule (DSCAM) Antibody (DS2-176); anti-DSCAM antibody
Host
Mouse
Reactivity
Chicken, Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
DS2-176
Specificity
A specific antibody against the chicken Dscam C-terminal region (amino acids 1207 to 1527).
Form/Format
Lyophilized protein G purified in PBS pH7.4
Sequence Length
103
Applicable Applications for anti-DSCAM antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
If reconstituted in 100 muL: IHC: 1:250-1:500.
Preservative
None
Preparation and Storage
Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).
For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Translected human embryonic kidney 293 cells, overexpressing chicken Dscam or mouse Dscam, fixed with 4% paralormaldehyde/PBS. Cells are labeled with anti-Dscam (DS2-176) antibody)
Testing Data
(Translected human embryonic kidney 293 cells, overexpressing chicken Dscam or mouse Dscam, fixed with 4% paralormaldehyde/PBS. Cells are labeled with anti-Dscam (DS2-176) antibody)
Related Product Information for anti-DSCAM antibody
Down syndrome cell adhesion molecule (Dscam) is a cell adhesion molecule that plays a role in neuronal self-avoidance. It promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Dscam mediates, within retinal amacrine and ganglion cell subtypes, both isoneuronal selfavoidance for creating an orderly dendritic arborization and heteroneuronal selfavoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. It is the receptor for netrin that is required for axon guidance independently of and in collaboration with the receptor DCC. In spinal cord development, it plays a role in guiding commissural axons during projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Dscam enhances netrin-induced phosphorylation of PAK1 and FYN. It mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. Dscam interacts with DCC, Netrin-1, PAK1 and RAC1.
References
1. Yamagata M and Sanes JR-Dscam and Sidekick proteins direct lamina-specific synaptic connections invertebrate retina. Transfected human embryonic kidney 293 cells, overexpressing chicken Dscam or mouse Dscam, fixed with 4% paraformaldehyde/PBS. Cells are labeled with anti-Dscam antibody.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
10,927 Da
NCBI Official Full Name
Dscam, partial
UniProt Protein Name
Dscam
Protein Family
UniProt Gene Name
dscam
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Product Notes
The DSCAM dscam (Catalog #AAA588022) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Down Syndrome Cell Adhesion Molecule (DSCAM) Antibody (DS2-176) reacts with Chicken, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Down Syndrome Cell Adhesion Molecule (DSCAM) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). If reconstituted in 100 muL: IHC: 1:250-1:500. Researchers should empirically determine the suitability of the DSCAM dscam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Down Syndrome Cell Adhesion Molecule (DSCAM), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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