Typical Testing Data/Standard Curve (for reference only)
Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit | SGPP2 elisa kit
Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit
Gene Names
SGPP2; SPP2; SPPase2
Reactivity
Human
Synonyms
Sphingosine-1-Phosphate Phosphatase 2 (SGPP2); Human Sphingosine-1-Phosphate Phosphatase 2 (SGPP2) ELISA Kit; SPP2; SGPP2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SGPP2. No significant cross-reactivity or interference between SGPP2 and analogues was observed.
Sequence Length
271
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.059ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SGPP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SGPP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SGPP2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SGPP2 in human tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SGPP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SGPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SGPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SGPP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SGPP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SGPP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SGPP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SGPP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,239 Da
NCBI Official Full Name
sphingosine-1-phosphate phosphatase 2 isoform b
NCBI Official Synonym Full Names
sphingosine-1-phosphate phosphatase 2
NCBI Official Symbol
SGPP2
NCBI Official Synonym Symbols
SPP2; SPPase2
NCBI Protein Information
sphingosine-1-phosphate phosphatase 2
UniProt Protein Name
Sphingosine-1-phosphate phosphatase 2
Protein Family
UniProt Gene Name
SGPP2
UniProt Synonym Gene Names
SPPase2; Spp2; hSPP2
NCBI Description
The protein encoded by this gene is a transmembrane protein that degrades the bioactive signaling molecule sphingosine 1-phosphate. The encoded protein is induced during inflammatory responses and has been shown to be downregulated by the microRNA-31 tumor suppressor. Alternative splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2016]
Uniprot Description
Has specific phosphohydrolase activity towards sphingoid base 1-phosphates. Has high phosphohydrolase activity against dihydrosphingosine-1-phosphate and sphingosine-1-phosphate (S1P) in vitro. May play a role in attenuating intracellular sphingosine 1-phosphate (S1P) signaling. May play a role in pro-inflammatory signaling.
Research Articles on SGPP2
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Product Notes
The Human SGPP2 sgpp2 (Catalog #AAA457169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457169 ELISA Kit recognizes Human SGPP2. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Phosphatase 2 (SGPP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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