Typical Testing Data/Standard Curve (for reference only)
Mouse Aryl hydrocarbon receptor, AhR ELISA Kit | AhR elisa kit
Mouse Aryl hydrocarbon receptor, AhR ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AhR ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AhR antibody and an AhR-HRP conjugate. The assay sample and buffer are incubated together with AhR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AhR concentration since AhR from samples and AhR-HRP conjugate compete for the anti-AhR antibody binding site. Since the number of sites is limited, as more sites are occupied by AhR from the sample, fewer sites are left to bind AhR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AhR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.
Protein type: Nuclear receptor; DNA-binding; Transcription factor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein dimerization activity; protein binding; signal transducer activity; ligand-dependent nuclear receptor activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; Hsp90 protein binding; aryl hydrocarbon receptor activity; transcription factor binding; transcription factor activity
Biological Process: prostate gland development; transcription from RNA polymerase II promoter; gland development; positive regulation of cell size; blood circulation; positive regulation of transcription, DNA-dependent; response to toxin; rhythmic process; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to organic cyclic substance; smooth muscle development; B cell homeostasis; regulation of transcription, DNA-dependent; ovarian follicle development; post-embryonic hemopoiesis; regulation of blood pressure; response to stress; regulation of B cell proliferation; T cell homeostasis; circadian regulation of gene expression; regulation of blood vessel size; positive regulation of transcriptional preinitiation complex assembly; positive regulation of growth; spleen development; blood vessel development; camera-type eye development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; immune system process; embryonic hemopoiesis; negative regulation of systemic arterial blood pressure; B-1 B cell homeostasis; liver development; cell cycle; negative regulation of vasoconstriction; patterning of blood vessels; regulation of transcription from RNA polymerase II promoter; response to xenobiotic stimulus; regulation of gene expression; xenobiotic metabolic process; B cell differentiation; blood vessel morphogenesis; blood vessel remodeling; negative regulation of transcription, DNA-dependent; lymphocyte homeostasis; reproductive structure development
Research Articles on AhR
Similar Products
Product Notes
The Mouse AhR ahr (Catalog #AAA720572) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720572 ELISA Kit recognizes Mouse AhR. It is sometimes possible for the material contained within the vial of "Aryl hydrocarbon receptor, AhR, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
