Rabbit anti-Human Aromatase Polyclonal Antibody | anti-CYP19A1 antibody

Anti-Aromatase antibody

Cat. Num. AAA175728

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: Aromatase; Polyclonal Antibody; Anti-Aromatase antibody; Cytochrome P450 19A1; cytochrome P450; family 19; subfamily A; polypeptide 1; ARO 1 antibody; ARO antibody; ARO1 antibody; Aromatase antibody; CP19A_HUMAN antibody; CPV antibody; CPV1 antibody; CYAR antibody; CYP19 antibody; CYP19A1 antibody; CYPXIX antibody; Cytochrome P-450AROM antibody; Cytochrome P450 19A1 antibody; Cytochrome P450 family 19 subfamily A polypeptide 1 antibody; Cytochrome P450; polypeptide 1 antibody; subfamily XIX(aromatization of androgens) antibody; Estrogen synthase antibody; Estrogen synthetase antibody; Flavoprotein linked monooxygenase antibody; MGC104309 antibody; Microsomal monooxygenase antibody; OTTHUMP00000162543 antibody; OTTHUMP00000198350 antibody; P 450AROM antibody; P450AROM antibody; anti-CYP19A1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 503

• Applicable Applications for anti-CYP19A1 antibody: Western Blot (WB)
• Application Notes: WB: The detection limit for Aromatase is approximately 1ng/lane under non-reducing and reducing conditions.; ; Other applications have not been tested.; optimal dilutions should be determined by end user.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase (485-503aa KNMLEMIFTPRNSDRCLEH).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Aromatase antibody, MBS175728, Western blottingLane 1: Human Placenta Tissue LysateLane 2: Human Placenta Tissue Lysate )

Immunohistochemistry (IHC)

(Anti-Aromatase antibody, MBS175728, IHC(P)IHC(P): Human Placenta Tissue )

Related Product Information for anti-CYP19A1 antibody

Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

NCBI and Uniprot Product Information

• NCBI GI #: 117293
• NCBI GeneID: 1588
• UniProt Accession #: P11511; Q16731; Q3B764; Q58FA0; Q8IYJ7
• NCBI Official Full Name: Aromatase
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)
• UniProt Protein Name: Aromatase
• Protein Family: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - androgen and estrogen; EC 1.14.14.14; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; steroid biosynthetic process; estrogen biosynthetic process; sterol metabolic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome