Rabbit anti-Human ARMET/MANF Polyclonal Antibody | anti-MANF antibody

Anti-ARMET/MANF Antibody, Rabbit Polyclonal

Cat. Num. AAA8105274

• Gene Names: MANF; ARP; ARMET
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: ARMET/MANF; Polyclonal Antibody; Anti-ARMET/MANF Antibody; Rabbit Polyclonal; ARMET/MANF Antibody; Rabbit PAb; Antigen Affinity Purified; mesencephalic astrocyte-derived neurotrophic factor; Anti-ARMET Antibody; Anti-ARP Antibody; anti-MANF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ARMET/MANF
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MANF antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; ICC/IF: 1:100-1:500
• Immunogen: Recombinant Human ARMET/MANF Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human ARMET/MANF (rh ARMET/MANF). ARMET/MANF specific IgG was purified by Human ARMET/MANF affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human MANF in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MANF in human pancreas with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MANF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MANF polyclonal antibody (dilution ratio 1:500) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Related Product Information for anti-MANF antibody

Mesencephalic astrocyte-derived neurotrophic factor, also known as Protein ARMET, Arginine-rich protein, MANF, and ARMET, is a secreted protein that belongs to the ARMET family. ARMET selectively promotes the survival of dopaminergic neurons of the ventral midbrain. It modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. ARMET enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. ARMET inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. The N-terminal region of ARMET may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. MANF reduces endoplasmic reticulum (ER) stress and has neurotrophic effects on dopaminergic neurons. Intracortical delivery of recombinant MANF protein protects tissue from ischemic brain injury. MANF has been described as a survival factor for dopaminergic neurons. MANF expression was widespread in the nervous system and non-neuronal tissues. In the brain, relatively high MANF levels were detected in the cerebral cortex, hippocampus, and cerebellar Purkinje cells. The widespread expression of MANF together with its evolutionary conserved nature and regulation by brain insults suggests that it has important functions both under normal and pathological conditions in many tissue types.

References

Shridhar V., et al., 1996, Oncogene 12:1931-1939. Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569. Petrova P., et al., 2003, J. Mol. Neurosci. 20:173-188. Lindholm, P. et al., 2008, Mol Cell Neurosci. 39 (3):356-71. Airavaara, M. et al., 2010, Exp Neurol. 225 (1):104-13.

NCBI and Uniprot Product Information

• NCBI GI #: 299523086
• NCBI GeneID: 7873
• NCBI Accession #: NP_006001.3
• NCBI GenBank Nucleotide #: NM_006010.4
• UniProt Accession #: P55145; Q14CX4; Q86U67; Q96IS4
• Molecular Weight: 20,700 Da
• NCBI Official Full Name: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Synonym Full Names: mesencephalic astrocyte-derived neurotrophic factor
• NCBI Official Symbol: MANF
• NCBI Official Synonym Symbols: ARP; ARMET
• NCBI Protein Information: mesencephalic astrocyte-derived neurotrophic factor; arginine-rich, mutated in early stage tumors
• UniProt Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Protein Family: Alpha-mannosidase
• UniProt Gene Name: MANF
• UniProt Synonym Gene Names: ARMET; ARP
• UniProt Entry Name: MANF_HUMAN

Uniprot Description

ARMET: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. Belongs to the ARMET family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular space; perinuclear region of cytoplasm; endoplasmic reticulum; nucleus

Molecular Function: growth factor activity

Biological Process: vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure; response to unfolded protein

Disease: Pancreatic Cancer