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Western Blot (WB) (staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Goat ARL4A Polyclonal Antibody | anti-ARL4A antibody

ARL4A (ADP-ribosylation Factor-like Protein 4A, ARL4)

Gene Names
ARL4A; ARL4
Reactivity
Human, Mouse, Porcine, Rat
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
ARL4A; Polyclonal Antibody; ARL4A (ADP-ribosylation Factor-like Protein 4A; ARL4); Anti -ARL4A (ADP-ribosylation Factor-like Protein 4A; anti-ARL4A antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Specificity
Recognizes human ARL4A. Species sequence homology: Mouse, porcine and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-ARL4A antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1000
Western Blot: 1-3ug/ml, human testis lysate observed on ~23kD bands
Immunogen
Synthetic peptide corresponding to GNGLSDQTSILSN-C, from human ARL4A, at N-terminal (NP_001032241.1, NP_005729.1, P_997625.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-ARL4A antibody
ARL4A, also known as ADP-ribosylation factor-like protein 4A, is a member of the ARF-like protein (ARL) subfamily of small GTPases. It is most closely related to ARL6 and ARL7. Unlike ARFs, ARL4 does not activate the cholera toxin ADP-ribosyltranferase. This protein may play a role in neurogenesis during embryonic development and somitogenesis in the early stages of adult spermatogenesis.
Product Categories/Family for anti-ARL4A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,615 Da
NCBI Official Full Name
ADP-ribosylation factor-like protein 4A
NCBI Official Synonym Full Names
ADP-ribosylation factor-like 4A
NCBI Official Symbol
ARL4A
NCBI Official Synonym Symbols
ARL4
NCBI Protein Information
ADP-ribosylation factor-like protein 4A; ADP-ribosylation factor-like 4
UniProt Protein Name
ADP-ribosylation factor-like protein 4A
UniProt Gene Name
ARL4A
UniProt Synonym Gene Names
ARL4
UniProt Entry Name
ARL4A_HUMAN

NCBI Description

ADP-ribosylation factor-like 4A is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4A is similar to ARL4C and ARL4D and each has a nuclear localization signal and an unusually high guaninine nucleotide exchange rate. ARL4A is located in both the nuclear and extranuclear cell compartments. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL4A: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Belongs to the small GTPase superfamily. Arf family.

Protein type: Nucleolus; G protein, monomeric, ARF; G protein, monomeric

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: cytoplasm; nucleolus; plasma membrane; nucleus

Molecular Function: protein binding; GTP binding

Biological Process: small GTPase mediated signal transduction; brown fat cell differentiation

Research Articles on ARL4A

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Product Notes

The ARL4A arl4a (Catalog #AAA6012262) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ARL4A (ADP-ribosylation Factor-like Protein 4A, ARL4) reacts with Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARL4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1000 Western Blot: 1-3ug/ml, human testis lysate observed on ~23kD bands. Researchers should empirically determine the suitability of the ARL4A arl4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARL4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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