Western Blot (WB)
((2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat ARIH2/TRIAD1 Polyclonal Antibody | anti-ARIH2 antibody
Goat anti-ARIH2/TRIAD1 Antibody
Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF.
Immunoprecipitation: This antibody works well in IP.
Western Blot (WB)
((2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]
Uniprot Description
ARIH2: E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys- 63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis. Belongs to the RBR family. Ariadne subfamily.
Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: cytoplasm; nucleus; ubiquitin ligase complex
Molecular Function: ligase activity; protein binding; ubiquitin conjugating enzyme binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: developmental cell growth; multicellular organismal development; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process
Research Articles on ARIH2
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Product Notes
The ARIH2 arih2 (Catalog #AAA421092) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARIH2/TRIAD1 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ARIH2/TRIAD1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. Immunoprecipitation: This antibody works well in IP. Researchers should empirically determine the suitability of the ARIH2 arih2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EQRRRTLLKD FHDT. It is sometimes possible for the material contained within the vial of "ARIH2/TRIAD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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