Rabbit Integrin beta1 ELISA Kit | ITGbeta1 elisa kit

Rabbit Integrin beta1 ELISA Kit

Cat. Num. AAA745503

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Reactivity: Rabbit
• Synonyms: Integrin beta1; Rabbit Integrin beta1 ELISA Kit; Rabbit Integrin b1 ELISA Kit; ITGbeta1 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This ITGbeta1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ITGbeta1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGbeta1 elisa kit

Principle of the assay: ITGbeta1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ITGbeta1 antibody and an ITGbeta1-HRP conjugate. The assay sample and buffer are incubated together with ITGbeta1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ITGbeta1 concentration since ITGbeta1 from samples and ITGbeta1-HRP conjugate compete for the anti-ITGbeta1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ITGbeta1 from the sample, fewer sites are left to bind ITGbeta1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITGbeta1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ITGbeta1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 19743823
• NCBI GeneID: 3688
• NCBI Accession #: NP_596867.1
• NCBI GenBank Nucleotide #: NM_133376.2
• Molecular Weight: 88,884 Da
• NCBI Official Full Name: integrin beta-1 isoform 1A
• NCBI Official Synonym Full Names: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
• UniProt Protein Name: Integrin beta-1
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA
• UniProt Entry Name: ITB1_HUMAN

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Cell surface

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: cell surface; focal adhesion; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; hemidesmosome; membrane; lamellipodium; cytoplasm; melanosome; plasma membrane; basement membrane; neuromuscular junction; integrin complex; sarcolemma; receptor complex; external side of plasma membrane; filopodium; cleavage furrow

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; actin binding; alpha-actinin binding; peptide binding; protein kinase binding; viral receptor activity; integrin binding; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding

Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; regulation of cell cycle; axon extension; positive regulation of apoptosis; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; negative regulation of neuron differentiation; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; sarcomere organization; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; stress fiber formation; calcium-independent cell-matrix adhesion; cellular defense response; leukocyte tethering or rolling; homophilic cell adhesion; blood coagulation; positive regulation of cell migration