Goat ARHU/WRCH1 Polyclonal Antibody | anti-RHOU antibody
Goat anti-ARHU/WRCH1 Antibody
Western Blot: Preliminary experiments gave an approx.22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011]
Uniprot Description
RHOU: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP- bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Belongs to the small GTPase superfamily. Rho family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Rho; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 1q42.11-q42.3
Cellular Component: cell projection; cytosol; focal adhesion; Golgi membrane; plasma membrane; podosome
Molecular Function: GTP binding; GTPase activity; metal ion binding; protein binding
Biological Process: actin cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; G1/S transition of mitotic cell cycle; intracellular protein transport; metabolic process; nucleocytoplasmic transport; Rac protein signal transduction; regulation of cell shape; regulation of small GTPase mediated signal transduction
Research Articles on RHOU
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Product Notes
The RHOU rhou (Catalog #AAA420847) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARHU/WRCH1 Antibody reacts with Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARHU/WRCH1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx.22kDa band in lysates of cell lines A431, HeLa and HEK293 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.2kDa according to NP_067028.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the RHOU rhou for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PPQQGDPAFP DRCEA. It is sometimes possible for the material contained within the vial of "ARHU/WRCH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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