Rabbit anti-Human, Mouse ARHGDIG Polyclonal Antibody | anti-ARHGDIG antibody

ARHGDIG Polyclonal Antibody

Cat. Num. AAA3006157

• Gene Names: ARHGDIG; RHOGDI-3
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARHGDIG; Polyclonal Antibody; ARHGDIG Polyclonal Antibody; Rho GDP-dissociation inhibitor 3; Rho GDI 3; Rho-GDI gamma; Rho GDIgamma; Rho-GDIgamma; anti-ARHGDIG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARHGDIG polyclonal antibody detects endogenous levels of ARHGDIG protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 225

• Applicable Applications for anti-ARHGDIG antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 176-221 of Human ARHGDIG.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARHGDIG polyclonal antibody at 1:500 dilutionLane1:Jurkat cell lysateLane2:Hela cell lysateLane3:Raw264. 7 cell lysate)

Related Product Information for anti-ARHGDIG antibody

The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTPbound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIalpha, Ly-GDI (also known as Rho GDIbeta and previously known as GDI/D4) and Rho GDIgamma. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 83313661
• NCBI GeneID: 398
• NCBI Accession #: NP_001167.2
• NCBI GenBank Nucleotide #: NP_001167.2
• UniProt Accession #: Q99819
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: rho GDP-dissociation inhibitor 3
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor gamma
• NCBI Official Symbol: ARHGDIG
• NCBI Official Synonym Symbols: RHOGDI-3
• NCBI Protein Information: rho GDP-dissociation inhibitor 3
• UniProt Protein Name: Rho GDP-dissociation inhibitor 3
• Protein Family: Rho GDP-dissociation inhibitor
• UniProt Gene Name: ARHGDIG
• UniProt Synonym Gene Names: Rho GDI 3
• UniProt Entry Name: GDIR3_HUMAN

NCBI Description

The GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases by inhibiting the exchange of GDP for GTP. See ARHGDIB (MIM 602843).[supplied by OMIM, Nov 2010]

Uniprot Description

RhoGDI gamma: Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post- translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP. Belongs to the Rho GDI family.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis; Inhibitor; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of protein localization; small GTPase mediated signal transduction; negative regulation of cell adhesion; Rho protein signal transduction; positive regulation of GTPase activity