Rabbit anti-Human, Mouse ARHGDIG Polyclonal Antibody | anti-ARHGDIG antibody

ARHGDIG Antibody

Cat. Num. AAA7044415

• Gene Names: ARHGDIG; RHOGDI-3
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: ARHGDIG; Polyclonal Antibody; ARHGDIG Antibody; Rho GDP-dissociation inhibitor 3Rho GDI 3; Rho-GDI gamma; anti-ARHGDIG antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 225

• Applicable Applications for anti-ARHGDIG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Species: Human
• Immunogen: Recombinant human Rho GDP-dissociation inhibitor 3 protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6910
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes :ARHGDIG antibody at 2 ug/mlLane 1 : Mouse brain tissueLane 2 : Mouse lung tissueSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 25 kDaObserved band size: 25,50 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS7044415 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7044415 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of mcf-7 cells using MBS7044415 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-ARHGDIG antibody

Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post-translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP.

NCBI and Uniprot Product Information

• NCBI GI #: 83313661
• NCBI GeneID: 398
• NCBI Accession #: NP_001167.2
• NCBI GenBank Nucleotide #: NM_001176.3
• UniProt Accession #: Q99819; Q4TT69; Q96S29
• Molecular Weight: 25,098 Da
• NCBI Official Full Name: rho GDP-dissociation inhibitor 3
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor gamma
• NCBI Official Symbol: ARHGDIG
• NCBI Official Synonym Symbols: RHOGDI-3
• NCBI Protein Information: rho GDP-dissociation inhibitor 3
• UniProt Protein Name: Rho GDP-dissociation inhibitor 3
• Protein Family: Rho GDP-dissociation inhibitor
• UniProt Gene Name: ARHGDIG
• UniProt Synonym Gene Names: Rho GDI 3
• UniProt Entry Name: GDIR3_HUMAN

NCBI Description

The GDP-dissociation inhibitors (GDIs) play a primary role in modulating the activation of GTPases by inhibiting the exchange of GDP for GTP. See ARHGDIB (MIM 602843).[supplied by OMIM, Nov 2010]

Uniprot Description

RhoGDI gamma: Inhibits GDP/GTP exchange reaction of RhoB. Interacts specifically with the GDP- and GTP-bound forms of post- translationally processed Rhob and Rhog proteins, both of which show a growth-regulated expression in mammalian cells. Stimulates the release of the GDP-bound but not the GTP-bound RhoB protein. Also inhibits the GDP/GTP exchange of RhoB but shows less ability to inhibit the dissociation of prebound GTP. Belongs to the Rho GDI family.

Protein type: Cell adhesion; G protein regulator, misc.; Motility/polarity/chemotaxis; Inhibitor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: protein binding

Biological Process: negative regulation of cell adhesion; regulation of small GTPase mediated signal transduction; Rho protein signal transduction