Rabbit ARHGAP30 Polyclonal Antibody | anti-ARHGAP30 antibody

ARHGAP30 Polyclonal Antibody

Cat. Num. AAA3007031

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ARHGAP30; Polyclonal Antibody; ARHGAP30 Polyclonal Antibody; Rho GTPase-activating protein 30; Rho-type GTPase-activating protein 30; anti-ARHGAP30 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARHGAP30 polyclonal antibody detects endogenous levels of ARHGAP30 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1101

• Applicable Applications for anti-ARHGAP30 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human ARHGAP30
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARHGAP30 polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:Raw264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-ARHGAP30 antibody

GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. ARHGAP30 (Rho GTPase activating protein 30), also known as Rho-type GTPase-activating protein 30, is a 1, 101 amino acid protein that contains one Rho-GAP domain and exists as four alternatively spliced isoforms. Conserved in chimpanzee, dog, cow, mouse and rat, ARHGAP30 is among the most neurite-enriched GAPs, which also includes ARHGAP21, FNBP2 and Bcr). One of four single-nucleotide polymorphisms (SNPs) at the USF-1 gene locus, rs2774279, which is located in the promoter area of the USF-1 gene, which is in turn located within its flanking gene ARHGAP30, is linked to low-density lipoprotein cholesterol levels, incident type 2 diabetes mellitus and increased cardiovascular risk.

NCBI and Uniprot Product Information

• NCBI GI #: 71040098
• NCBI GeneID: 257106
• NCBI Accession #: NP_001020769.1
• NCBI GenBank Nucleotide #: NP_001020769.1
• UniProt Accession #: Q7Z6I6
• Molecular Weight: ~ 119kDa
• NCBI Official Full Name: rho GTPase-activating protein 30 isoform 1
• NCBI Official Synonym Full Names: Rho GTPase activating protein 30
• NCBI Official Symbol: ARHGAP30
• NCBI Protein Information: rho GTPase-activating protein 30
• UniProt Protein Name: Rho GTPase-activating protein 30
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP30
• UniProt Entry Name: RHG30_HUMAN

Uniprot Description

ARHGAP30: GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: intracellular membrane-bound organelle; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity