Western Blot (WB)
(Western blot analysis of ARHGAP25 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ARHGAP25 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse ARHGAP25 Polyclonal Antibody | anti-ARHGAP25 antibody
ARHGAP25, ID (ARHGAP25, KIAA0053, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25) (PE)
Gene Names
ARHGAP25; KAIA0053; HEL-S-308
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ARHGAP25; Polyclonal Antibody; ID (ARHGAP25; KIAA0053; Rho GTPase-activating protein 25; Rho-type GTPase-activating protein 25) (PE); Rho-type GTPase-activating protein 25; anti-ARHGAP25 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-ARHGAP25 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ARHGAP25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192~221 amino acids from the Central region of human ARHGAP25.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ARHGAP25 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ARHGAP25 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ARHGAP25 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ARHGAP25 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(ARHGAP25 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(ARHGAP25 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGAP25 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ARHGAP25 antibody
ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration.
Product Categories/Family for anti-ARHGAP25 antibody
References
Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009). Katoh, M., et al. Int. J. Mol. Med. 14(2):333-338(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
645
NCBI Official Full Name
Rho GTPase-activating protein 25
NCBI Official Synonym Full Names
Rho GTPase activating protein 25
NCBI Official Symbol
ARHGAP25
NCBI Official Synonym Symbols
KAIA0053; HEL-S-308
NCBI Protein Information
rho GTPase-activating protein 25; epididymis secretory protein Li 308; rho-type GTPase-activating protein 25
UniProt Protein Name
Rho GTPase-activating protein 25
Protein Family
UniProt Gene Name
ARHGAP25
UniProt Synonym Gene Names
KIAA0053
UniProt Entry Name
RHG25_HUMAN
NCBI Description
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Uniprot Description
ARHGAP25: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: cytosol
Molecular Function: GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
Research Articles on ARHGAP25
Similar Products
Product Notes
The ARHGAP25 arhgap25 (Catalog #AAA6274895) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP25, ID (ARHGAP25, KIAA0053, Rho GTPase-activating protein 25, Rho-type GTPase-activating protein 25) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP25 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-100 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARHGAP25 arhgap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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