Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PSMA4 Polyclonal Antibody | anti-PSMA4 antibody
Goat anti-PSMA4 Antibody
Western Blot: Approx 28kDa band observed in lysates of cell lines K562, NIH3T3 and in lysates of Human Tonsil, Human Peripheral Blood Mononucleocytes and in Mouse and Rat Spleen (calculated MW of 29.5kDa according to NP_002780.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PSMA4: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Proteasome complex; EC 3.4.25.1; Protease
Chromosomal Location of Human Ortholog: 15q25.1
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus; proteasome complex; proteasome core complex
Molecular Function: protein binding; threonine endopeptidase activity
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; viral reproduction; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on PSMA4
Similar Products
Product Notes
The PSMA4 psma4 (Catalog #AAA422448) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PSMA4 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PSMA4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 28kDa band observed in lysates of cell lines K562, NIH3T3 and in lysates of Human Tonsil, Human Peripheral Blood Mononucleocytes and in Mouse and Rat Spleen (calculated MW of 29.5kDa according to NP_002780.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the PSMA4 psma4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDANVLTNEL R. It is sometimes possible for the material contained within the vial of "PSMA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
