Rabbit anti-Mouse Argonaute-2 Polyclonal Antibody | anti-AGO2 antibody

Anti-Argonaute-2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111905

• Gene Names: Ago2; Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
• Reactivity: Mouse
• Applications: ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Argonaute-2; Polyclonal Antibody; Anti-Argonaute-2 Antibody; Rabbit Polyclonal; AGO2/Argonaute 2/EIF2C2 Antibody; Rabbit PAb; Antigen Affinity Purified; argonaute RISC catalytic component 2; Anti-1110029L17Rik Antibody; Anti-2310051F07Rik Antibody; Anti-Ago2 Antibody; Anti-AI225898 Antibody; Anti-AL022874 Antibody; Anti-AW546247 Antibody; Anti-Eif2c2 Antibody; Anti-ENSMUSG00000072493 Antibody; Anti-Gerp95 Antibody; Anti-Gm10365 Antibody; Anti-KIAA4215 Antibody; Anti-mKIAA4215 Antibody; anti-AGO2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse AGO2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-AGO2 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Immunogen: Recombinant Mouse AGO2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse AGO2 (rM AGO2). AGO2 specific IgG was purified by Mouse AGO2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-AGO2 antibody

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

References

O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.

NCBI and Uniprot Product Information

• NCBI GI #: 219842353
• NCBI GeneID: 239528
• NCBI Accession #: NP_694818.3
• NCBI GenBank Nucleotide #: NM_153178.4
• UniProt Accession #: Q8CJG0; Q4VAB3; Q571J6; A1A563
• Molecular Weight: 97,304 Da
• NCBI Official Full Name: protein argonaute-2
• NCBI Official Synonym Full Names: argonaute RISC catalytic subunit 2
• NCBI Official Symbol: Ago2
• NCBI Official Synonym Symbols: Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik; ENSMUSG00000072493
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: Ago2
• UniProt Synonym Gene Names: Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2
• UniProt Entry Name: AGO2_MOUSE

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: EC 3.1.26.n2; RNA processing; RNA-binding

Cellular Component: cytoplasm; cytosol; dendrite; membrane; mitochondrion; mRNA cap complex; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endonuclease activity; endoribonuclease activity; hydrolase activity; metal ion binding; miRNA binding; mRNA binding; nuclease activity; nucleic acid binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; RNA binding; single-stranded RNA binding; siRNA binding

Biological Process: cell differentiation; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; multicellular organismal development; negative regulation of translational initiation; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; pre-microRNA processing; regulation of transcription, DNA-dependent; regulation of translation; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; transcription, DNA-dependent