AGO2 / Argonaute 2 / EIF2C2 Recombinant Protein | AGO2 recombinant protein

Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag)

Cat. Num. AAA2546853

• Gene Names: Ago2; Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: AGO2 / Argonaute 2 / EIF2C2; Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag); 1110029L17Rik; 2310051F07Rik; AI225898; AL022874; AW546247; Eif2c2; ENSM USG00000072493; Gerp95; Gm10365; mKIAA4215; AGO2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween 80.
• Sequence: Met 1-Ala 860

• Application Notes: The recombinant mouse AGO2 consists of 878 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
• Species: Mouse
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method. Lyophilized
• Reconstitution: It is recommended that sterile water (70uL) be added to the vial to prepare a stock solution of 0.15mg/mL. Concentration is measured by UV-Vis.
• Tag: N-his
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for AGO2 recombinant protein

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.

NCBI and Uniprot Product Information

• NCBI GI #: 219842353
• NCBI GeneID: 239528
• NCBI Accession #: NP_694818.3
• NCBI GenBank Nucleotide #: NM_153178.4
• UniProt Accession #: Q8CJG0; Q4VAB3; Q571J6; A1A563
• Molecular Weight: Molecular Mass: 99 kDa; Actual Protein Molecolar Mass: 105 kDa
• NCBI Official Full Name: protein argonaute-2
• NCBI Official Synonym Full Names: argonaute RISC catalytic subunit 2
• NCBI Official Symbol: Ago2
• NCBI Official Synonym Symbols: Eif2c2; Gerp95; Gm10365; AI225898; AL022874; AW546247; mKIAA4215; 1110029L17Rik; 2310051F07Rik
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: Ago2
• UniProt Synonym Gene Names: Eif2c2; Kiaa4215; Argonaute2; mAgo2; eIF-2C 2; eIF2C 2

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: EC 3.1.26.n2; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 15|15 D3

Cellular Component: cytoplasm; cytosol; dendrite; membrane; mitochondrion; mRNA cap complex; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; miRNA binding; mRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; RNA binding; single-stranded RNA binding; siRNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; pre-microRNA processing; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing