Rabbit anti-Human ARG2 Polyclonal Antibody | anti-ARG2 antibody

ARG2 Polyclonal Antibody

Cat. Num. AAA3007450

• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: ARG2; Polyclonal Antibody; ARG2 Polyclonal Antibody; Arginase-2; mitochondrial; Kidney-type arginase; Non-hepatic arginase; Type II arginase; anti-ARG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARG2 polyclonal antibody detects endogenous levels of ARG2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 354

• Applicable Applications for anti-ARG2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 310-354 of Human ARG2
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ARG2 polyclonal antibody at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug))

Related Product Information for anti-ARG2 antibody

Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea. The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 4502215
• NCBI GeneID: 384
• NCBI Accession #: NP_001163.1
• NCBI GenBank Nucleotide #: NP_001163.1
• UniProt Accession #: P78540
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: arginase-2, mitochondrial
• NCBI Official Synonym Full Names: arginase 2
• NCBI Official Symbol: ARG2
• NCBI Protein Information: arginase-2, mitochondrial
• UniProt Protein Name: Arginase-2, mitochondrial
• Protein Family: Arginase
• UniProt Gene Name: ARG2
• UniProt Entry Name: ARGI2_HUMAN

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ARG2: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders. Belongs to the arginase family.

Protein type: Hydrolase; Mitochondrial; Amino Acid Metabolism - arginine and proline; EC 3.5.3.1

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: metal ion binding; arginase activity

Biological Process: striated muscle contraction; arginine metabolic process; ureteric bud development; nitric oxide biosynthetic process; urea cycle