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Western Blot (WB) (Western Blot analysis of ARG1 on different lysates using anti-ARG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat liver Lane 2: Human liver)

Rabbit ARG1 Polyclonal Antibody | anti-ARG1 antibody

ARG1 Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
ARG1; Polyclonal Antibody; ARG1 Polyclonal Antibody; arg1; ARGI1; Arginase-1; Liver-type arginase; Type I arginase; anti-ARG1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ARG1 polyclonal antibody detects endogenous levels of ARG1 protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
322
Applicable Applications for anti-ARG1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:1,000-1:2,000
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of ARG1 on different lysates using anti-ARG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat liver Lane 2: Human liver)

Western Blot (WB) (Western Blot analysis of ARG1 on different lysates using anti-ARG1 antibody at 1/1,000 dilution. Positive control: Lane 1: Rat liver Lane 2: Human liver)
Related Product Information for anti-ARG1 antibody
Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea. The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
383
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35/25kDa
NCBI Official Full Name
arginase-1 isoform 2
NCBI Official Synonym Full Names
arginase 1
NCBI Official Symbol
ARG1
NCBI Protein Information
arginase-1
UniProt Protein Name
Arginase-1
Protein Family
UniProt Gene Name
ARG1
UniProt Entry Name
ARGI1_HUMAN

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; EC 3.5.3.1

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; liver development; response to amino acid stimulus; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; response to amine stimulus; urea cycle; lung development

Disease: Argininemia

Research Articles on ARG1

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Product Notes

The ARG1 arg1 (Catalog #AAA3012256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARG1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1,000-1:2,000. Researchers should empirically determine the suitability of the ARG1 arg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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