GFPT1 recombinant protein

GFPT1 Protein, Human, Recombinant

Cat. Num. AAA8120669

• Gene Names: GFPT1; GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L
• Purity: >97% as determined by SDS-PAGE
• Synonyms: GFPT1; GFPT1 Protein; Human; Recombinant; Human GFPT1/GFAT Protein; CMSTA1 Protein; GFA Protein; GFAT Protein; GFAT1 Protein; GFAT1m Protein; GFPT Protein; GFPT1L Protein; MSLG Protein; glutamine--fructose-6-phosphate transaminase 1; GFPT1 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln332-Glu699

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: The sequence corresponding to amino acids (Gln 332-Glu 699) of human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GFPT1 recombinant protein

Background: Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.

References

McKnight G.L., et al.,(1992), Molecular cloning, cDNA sequence, and bacterial expression of human glutamine:fructose-6-phosphate amidotransferase. J. Biol. Chem. 267:25208-25212.Hillier L.W., et al., (2005), Generation and annotation of the DNA sequences of human chromosomes 2 and 4.Nature 434:724-731.Beausoleil S.A., et al.,(2006), A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat. Biotechnol. 24:1285-1292.

NCBI and Uniprot Product Information

• NCBI GI #: 183082
• NCBI GeneID: 2673
• Molecular Weight: 76,759 Da
• NCBI Official Full Name: glutamine:fructose-6-phosphate amidotransferase
• NCBI Official Synonym Full Names: glutamine--fructose-6-phosphate transaminase 1
• NCBI Official Symbol: GFPT1
• NCBI Official Synonym Symbols: GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L
• NCBI Protein Information: glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
• Protein Family: Glutamine--fructose-6-phosphate aminotransferase

NCBI Description

This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]