Rabbit anti-Mouse ARF, p19 Polyclonal Antibody | anti-Arf1 antibody

ARF, p19

Cat. Num. AAA630135

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: ARF; p19; Polyclonal Antibody; Anti -ARF; anti-Arf1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Specific for p19ARF. Species crossreactivity: Reacts with mouse p19ARF. Does not react with rat and human.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Antisera was eluted with 100 mM glycine, pH 2.5, and dialyzed against PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-Arf1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: Western Blot: 0.5-1ug/ml (ECL); Immunohistochemistry:Not determined; ELISA (indirect): 1:5,000
• Immunogen: KLH coupled peptide containing amino acids 54-75 of mouse p19ARF.
• Preparation and Storage: -20 degree C

Related Product Information for anti-Arf1 antibody

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop (3). These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.

By Western blot, this antibody recognizes IVT murine p19ARF and a clear band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs (1). The antibody can be competed from recognizing p19ARF using the antigenic peptide at 10ug/ml.

Product Categories/Family for anti-Arf1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 194353962
• NCBI GeneID: 11840
• NCBI Accession #: NP_001123880.1
• NCBI GenBank Nucleotide #: NM_001130408.1
• UniProt Accession #: P84078; P10947; P32889; Q3THZ2; Q3U849; Q3UDG1; Q3UF76
• Molecular Weight: 20,697 Da
• NCBI Official Full Name: ADP-ribosylation factor 1
• NCBI Official Synonym Full Names: ADP-ribosylation factor 1
• NCBI Official Symbol: Arf1
• NCBI Protein Information: ADP-ribosylation factor 1; OTTMUSP00000006686
• UniProt Protein Name: ADP-ribosylation factor 1
• Protein Family: Auxin response factor
• UniProt Gene Name: Arf1
• UniProt Entry Name: ARF1_MOUSE

Uniprot Description

ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein; G protein, monomeric, ARF

Cellular Component: peroxisomal membrane; Golgi apparatus; neuron projection; focal adhesion; cell; endomembrane system; trans-Golgi network; cytosol; Golgi membrane; COPI-coated vesicle; postsynaptic membrane; sarcomere; membrane; cytoplasm; late endosome; plasma membrane; synapse; intracellular; cell junction

Molecular Function: protein binding; GDP binding; protein homodimerization activity; GTP binding; magnesium ion binding; nucleotide binding

Biological Process: positive regulation of catalytic activity; vesicle-mediated transport; Golgi to transport vesicle transport; positive regulation of calcium ion-dependent exocytosis; protein transport; cellular copper ion homeostasis; positive regulation of protein secretion; transport; regulation of receptor internalization; small GTPase mediated signal transduction; actin filament organization; positive regulation of endocytosis