Rabbit anti-Human Arachidonate 15-lipoxygenase B Polyclonal Antibody | anti-ALOX15B antibody

Rabbit anti-human Arachidonate 15-lipoxygenase B polyclonal Antibody(ALOX15B)

Cat. Num. AAA1494742

• Gene Names: ALOX15B; 15-LOX-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Arachidonate 15-lipoxygenase B; Polyclonal Antibody; Rabbit anti-human Arachidonate 15-lipoxygenase B polyclonal Antibody(ALOX15B); 15-LOX-B; 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb; ALOX15B; anti-ALOX15B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 647

• Applicable Applications for anti-ALOX15B antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-1:2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Arachidonate 15-lipoxygenase B protein(1-200aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46048 / sc-46049 / sc-46050 / sc-67142
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Arachidonate 15-lipoxygenase B antibody at 11ug/mlLane 1:MCF-7 whole cell lysateLane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 76,68,70,73 kDaObserved band size: 76 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skin using MBS1494742 at dilution 1:100)

Related Product Information for anti-ALOX15B antibody

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.

NCBI and Uniprot Product Information

• NCBI GI #: 85067499
• NCBI GeneID: 247
• NCBI Accession #: NP_001034219.1
• NCBI GenBank Nucleotide #: NM_001039130.1
• UniProt Accession #: O15296; Q8IYQ2; Q8TEV3; Q8TEV4; Q8TEV5; Q8TEV6; Q9UKM4; D3DTR2
• Molecular Weight: 72,523 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase B isoform a
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase, type B
• NCBI Official Symbol: ALOX15B
• NCBI Official Synonym Symbols: 15-LOX-2
• NCBI Protein Information: arachidonate 15-lipoxygenase B
• UniProt Protein Name: Arachidonate 15-lipoxygenase B
• Protein Family: Arachidonate 15-lipoxygenase
• UniProt Gene Name: ALOX15B
• UniProt Synonym Gene Names: 15-LOX-B; 15-LOX-2
• UniProt Entry Name: LX15B_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.13.11.33; Cell cycle regulation; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; nucleus; plasma membrane

Molecular Function: arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipid binding; lipoxygenase activity

Biological Process: apoptosis; arachidonic acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of growth; positive regulation of keratinocyte differentiation; prostate gland development; regulation of epithelial cell differentiation