Rabbit anti-Human, Mouse FAM105B Polyclonal Antibody | anti-FAM105B antibody

FAM105B, ID (FAM105B, Protein FAM105B) (Biotin)

Cat. Num. AAA6297715

• Gene Names: OTULIN; GUM; FAM105B
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FAM105B; Polyclonal Antibody; ID (FAM105B; Protein FAM105B) (Biotin); Protein FAM105B; anti-FAM105B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FAM105B antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: FAM105B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-137 amino acids from the Central region of human FAM105B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FAM105B Antibody (Center) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the FAM105B antibody detected FAM105B protein (arrow).)

Immunohistochemistry (IHC)

(FAM105B Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FAM105B Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(FAM105B Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FAM105B antibody

Antibodies; FAM

References

Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 115334675
• NCBI GeneID: 90268
• NCBI Accession #: NP_612357.4
• NCBI GenBank Nucleotide #: NM_138348.4
• UniProt Accession #: Q96BN8; Q8NAS0; Q96IA3; D3DTD3
• Molecular Weight: 40,263 Da
• NCBI Official Full Name: ubiquitin thioesterase otulin
• NCBI Official Synonym Full Names: OTU deubiquitinase with linear linkage specificity
• NCBI Official Symbol: OTULIN
• NCBI Official Synonym Symbols: GUM; FAM105B
• NCBI Protein Information: ubiquitin thioesterase otulin; gumby; protein FAM105B; ubiquitin thioesterase Gumby; deubiquitinating enzyme otulin; family with sequence similarity 105, member B; OTU domain-containing deubiquitinase with linear linkage specificity
• UniProt Protein Name: Ubiquitin thioesterase otulin
• UniProt Gene Name: OTULIN
• UniProt Synonym Gene Names: FAM105B
• UniProt Entry Name: OTUL_HUMAN