Rabbit APRIL Polyclonal Antibody | anti-ANP32B antibody

APRIL, ID (Tumor Necrosis Factor Ligand Superfamily Member 13, TALL-2, TRDL-1, TNFSF13, CD256, TALL2, ZTNF2, UNQ383/PRO715)

Cat. Num. AAA648930

• Gene Names: ANP32B; APRIL; SSP29; PHAPI2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: APRIL; Polyclonal Antibody; ID (Tumor Necrosis Factor Ligand Superfamily Member 13; TALL-2; TRDL-1; TNFSF13; CD256; TALL2; ZTNF2; UNQ383/PRO715); Anti -APRIL; anti-ANP32B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APRIL. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-ANP32B antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:500; ELISA: 1:10,000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human APRIL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from HeLa/COLO205 cells, using TALL-2. The lane on the right is treated with the synthesized peptide.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells, using TALL-2. The picture on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-ANP32B antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 5454088
• NCBI GeneID: 10541
• NCBI Accession #: NP_006392.1
• NCBI GenBank Nucleotide #: NM_006401.2
• UniProt Accession #: Q92688; O00655; P78458; P78459; B2R9C7
• Molecular Weight: 28,788 Da
• NCBI Official Full Name: acidic leucine-rich nuclear phosphoprotein 32 family member B
• NCBI Official Synonym Full Names: acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• NCBI Official Symbol: ANP32B
• NCBI Official Synonym Symbols: APRIL; SSP29; PHAPI2
• NCBI Protein Information: acidic leucine-rich nuclear phosphoprotein 32 family member B; silver-stainable protein SSP29; acidic protein rich in leucines; putative HLA-DR-associated protein I-2
• UniProt Protein Name: Acidic leucine-rich nuclear phosphoprotein 32 family member B
• Protein Family: Acidic leucine-rich nuclear phosphoprotein 32 family
• UniProt Gene Name: ANP32B
• UniProt Synonym Gene Names: APRIL; PHAPI2; PHAPI2
• UniProt Entry Name: AN32B_HUMAN

Uniprot Description

Function: Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity

By similarity. Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. Ref.15

Subunit structure: Monomer. Interacts with histones H3 and H4. Ref.15

Subcellular location: Isoform 1: Nucleus. Note: Accumulates in the nuclei at the S phase

By similarity.Isoform 2: Cytoplasm. Note: Lacks a nuclear localization signal.

Tissue specificity: Expressed in heart, lung, pancreas, prostate and in spleen, thymus and placenta. Ref.3

Domain: Histone binding is mediated by the concave surface of the LRR region.

Post-translational modification: Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group

By similarity.

Sequence similarities: Belongs to the ANP32 family.Contains 4 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.