ApoScreen Annexin V Polyclonal Antibody | anti-ANXA5 antibody
Aposcreen Annexin V-UNLB
Annexin V-FITC: is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8 degree C.; protect from light.
Annexin V-BIOT: Store at 2-8 degree C; protect from light.
A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface.1 Annexin V (Mr 36-kDa), a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers.2,3. When PS is exposed on the extracellular face of a cell membrane, Annexin V binds with an affinity of approximately 7 nM4. Fluorochrome-conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry,5-8 thereby providing a convenient tool for detection of apoptotic cells.
Annexin V-FITC is a labeled Annexin V that can be used to evaluate subpopulations of cells along the apoptotic pathway without counterstaining with propidium iodide or other DNA dyes. Analysis is performed on a flow cytometer equipped with a 488 nm argon laser and gated on viable cells by light scatter (see flow histograms below). Alternatively, the cells can be examined by fluorescence microscopy.
2. Tait, J.F., et al. 1989. J. Biol. Chem. 264:7944.
3. Andree, H.A., et al. 1990. J. Biol. Chem. 265:4923.
4. Thiagarajan, P., et al. 1990. J. Biol. Chem. 265:17420.
5. Dachary, P.J., et al. 1993. Blood 81:2554.
6. Koopman, G., et al. 1994. Blood 84:1415.
7. Martin, S.J., et al. 1995. J. Exp. Med. 182:1545.
8. Wood, B.L., et al. 1996. Blood 88:1873
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]
Uniprot Description
Function: This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Subunit structure: Monomer. Binds ATRX and EIF5B
By similarity. Interacts with hepatitis B virus (HBV). Ref.16
Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.
Research Articles on ANXA5
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Product Notes
The ANXA5 anxa5 (Catalog #AAA670174) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ApoScreen Annexin V can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; FM. Researchers should empirically determine the suitability of the ANXA5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ApoScreen Annexin V, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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