Rabbit anti-Rat Apolipoprotein A I Polyclonal Antibody | anti-Apoa1 antibody

Anti-Apolipoprotein A I Antibody

Cat. Num. AAA177794

• Gene Names: Apoa1; apoA-I
• Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Apolipoprotein A I; Polyclonal Antibody; Anti-Apolipoprotein A I Antibody; Apolipoprotein A-I; Apo AI; Apo-AI; ApoA I; ApoA-I; APOA1; APOA1/APOC3 fusion gene; APOA1_HUMAN; Apolipoprotein A I precursor; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein of high density lipoprotein; ApolipoproteinAI; Brp14; Ltw1; Lvtw1; MGC117399; Sep1; Sep2; apolipoprotein A-I; anti-Apoa1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized; Contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

• Applicable Applications for anti-Apoa1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: ; Concentration: 0.1-0.5ug/ml ; Tested Species: Rat; Antigen Retrieval: -; ; IHC-P: ; Concentration: 0.5-1ug/ml; Tested Species: Rat; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. ; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: E. coli-derived rat Apolipoprotein A I recombinant protein (Position: D25-A259). Rat Apolipoprotein A I shares 61.7% and 68.6% amino acid (aa) sequence identity with human and mouse Apolipoprotein A I, respectively.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References

1. Brewer, H. B., Jr.; Fairwell, T.; LaRue, A.; Ronan, R.; Houser, A.; Bronzert, T. J. : The amino acid sequence of human apoA-I, an apolipoprotein isolated from high density lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004). "Role of apolipoprotein A-I in protecting against endotoxin toxicity". Acta Biochim. Biophys. Sin. (Shanghai) 36 (6): 419-24. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988). "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I". J. Clin. Invest. 82 (3): 803-7. 4. Zhang, Y.; Zanotti, I.; Reilly, M. P.; Glick, J. M.; Rothblat, G. H.; Rader, D. J. : Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 6978515
• NCBI GeneID: 25081
• NCBI Accession #: NP_036870.1
• NCBI GenBank Nucleotide #: NM_012738.1
• UniProt Accession #: P04639; O08877; O09054; Q5EBB2
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_RAT

NCBI Description

major component of high density lipoprotein (HDL) that is involved in intercellular cholesterol transport in astrocytes; cofactor for lecithin cholesterolacyltransferase (LCAT) [RGD, Feb 2006]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: G protein, monomeric, non-Rab; Cell development/differentiation; Transporter; Inhibitor; Endoplasmic reticulum; Vesicle; Motility/polarity/chemotaxis; Lipid-binding; Secreted; Secreted, signal peptide

Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; endocytic vesicle; extracellular region; extracellular space; nucleus

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; high-density lipoprotein binding; identical protein binding; lipase inhibitor activity; lipid binding; lipid transporter activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity

Biological Process: adrenal gland development; axon regeneration; axon regeneration in the peripheral nervous system; blood vessel endothelial cell migration; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipoprotein biosynthetic process; lipoprotein metabolic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of hydrolase activity; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phosphatidylcholine metabolic process; phospholipid efflux; phospholipid homeostasis; phospholipid metabolic process; phospholipid transport; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein amino acid oxidation; protein localization; protein stabilization; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; regulation of protein amino acid phosphorylation; response to drug; response to estrogen stimulus; response to nutrient; reverse cholesterol transport; sequestering of lipid; transforming growth factor beta receptor signaling pathway; triacylglycerol catabolic process; vitamin transport