Rabbit alpha-Tubulin Polyclonal Antibody | anti-TUBA1A antibody

alpha-Tubulin Antibody

Cat. Num. AAA154440

• Gene Names: TUBA1A; LIS3; TUBA3; B-ALPHA-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: alpha-Tubulin; Polyclonal Antibody; alpha-Tubulin Antibody; Tubulin alpha-1A; TUBA1A; TUBA3; LIS3; tubulin; alpha 1a; anti-TUBA1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: alpha-Tubulin antibody is human, mouse and rat reactive.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBA1A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: Western blot at 1 - 2 mug/ml.; Immunohistochemistry starting at 2.5 mug/mL.; Immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: alpha-Tubulin antibody was raised against an 18 amino acid peptide near the carboxy terminus of human alpha-Tubulin
• Buffer: alpha-Tubulin antibody is supplied in PBS containing 0.02% sodium azide.
• Species: Human
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of alpha-Tubulin in 293, Daudi, Hela, HepG2, Jurkat, K562, NH3T3, Raji, Ramos, U937, Human brain, Mouse brain, Rat Brain, Rabbit Brain, Rabbit Spleen, Zebrafish, Mouse Liver and Chicken liver lysate at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 2.5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 20 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of alpha-Tubulin in human brain tissue with alpha-Tubulin antibody at 5 μg/mL.)

Related Product Information for anti-TUBA1A antibody

alpha-Tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-Tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-Tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-Tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

Product Categories/Family for anti-TUBA1A antibody

Homeostasis

References

1. McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33. 2. Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53. 3. Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.

NCBI and Uniprot Product Information

• NCBI GI #: 37492
• NCBI GeneID: 7846
• UniProt Accession #: Q71U36; P04687; P05209; A8K0B8; G3V1U9
• Molecular Weight: Predicted: 50 kDa; Observed: 54 kDa
• NCBI Official Full Name: alpha-tubulin
• NCBI Official Synonym Full Names: tubulin alpha 1a
• NCBI Official Symbol: TUBA1A
• NCBI Official Synonym Symbols: LIS3; TUBA3; B-ALPHA-1
• NCBI Protein Information: tubulin alpha-1A chain
• UniProt Protein Name: Tubulin alpha-1A chain
• Protein Family: Alpha-tubulin N-acetyltransferase
• UniProt Gene Name: TUBA1A
• UniProt Synonym Gene Names: TUBA3

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: cytosol; microtubule; microtubule cytoskeleton; nucleus; recycling endosome

Molecular Function: protein binding; structural molecule activity

Biological Process: cell division; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Lissencephaly 3