Rabbit anti-Rat ApoA1 Polyclonal Antibody | anti-ApoA1 antibody

ApoA1 Antibody

Cat. Num. AAA840713

• Gene Names: Apoa1; apoA-I
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: ApoA1; Polyclonal Antibody; ApoA1 Antibody; Apolipoprotein A-1; Apo-A1; ApoA-1;; anti-ApoA1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-rat A1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 259

• Applicable Applications for anti-ApoA1 antibody: Western Blot (WB)
• Application Notes: Western blot analysis (0.5-4 ug/ml). Recombinant rat ApoA1 can be use as a positive control. However, the optimal conditions should be determined individually. Other applications have not been determined.
• Immunogen: E. coli expressed recombinant rat ApoA1
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-ApoA1 antibody

Background: ApoA1 is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. ApoA1 which is found exclusively in HDL has a unique ability to capture and solubilize free cholesterol. This ApoA-I ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is thomught to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of ApoA-I (called ApoA1 Milano). Rat ApoA1 s a 27.4 kDa protein of 235 amino acid residues.

NCBI and Uniprot Product Information

• NCBI GI #: 6978515
• NCBI GeneID: 25081
• NCBI Accession #: NP_036870.1
• NCBI GenBank Nucleotide #: NM_012738.1
• UniProt Accession #: P04639; O08877; O09054; Q5EBB2
• Molecular Weight: 30,062 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_RAT

NCBI Description

major component of high density lipoprotein (HDL) that is involved in intercellular cholesterol transport in astrocytes; cofactor for lecithin cholesterolacyltransferase (LCAT) [RGD, Feb 2006]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Inhibitor; Cell development/differentiation; Endoplasmic reticulum; Motility/polarity/chemotaxis; Lipid-binding; Transporter; Vesicle; G protein, monomeric, non-Rab; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; extracellular region; cytoplasmic vesicle; nucleus; vesicle

Molecular Function: lipid transporter activity; identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; enzyme binding; phospholipid transporter activity; cholesterol transporter activity; chemorepellent activity; phospholipid binding; lipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; protein localization; sequestering of lipid; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; negative regulation of hydrolase activity; positive regulation of stress fiber formation; response to drug; cholesterol metabolic process; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; axon regeneration; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; phospholipid transport; blood vessel endothelial cell migration; phosphatidylcholine metabolic process; phospholipid efflux; phospholipid metabolic process; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; regulation of protein amino acid phosphorylation; cholesterol transport; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; protein stabilization; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; negative regulation of inflammatory response; endothelial cell proliferation