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Western Blot (WB) (Western Blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.)

Rabbit anti-Human APOC3 Polyclonal Antibody | anti-APOC3 antibody

APOC3 Polyclonal Antibody

Gene Names
APOC3; APOCIII
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified.
Synonyms
APOC3; Polyclonal Antibody; APOC3 Polyclonal Antibody; Apo-CIII; ApoC-III; APO C3; Apo CIII; APOC 3; ApoC III; ApoCIII; Apolipoprotein C III; Apolipoprotein C-III; Apolipoprotein C3; ApolipoproteinCIII; MGC150353; anti-APOC3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
APOC3 polyclonal antibody detects endogenous levels of APOC3 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
99
Applicable Applications for anti-APOC3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein with Human APOC3 aa 1-100.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.)

Western Blot (WB) (Western Blot analysis of APOC3 on human liver tissue lysate using anti-APOC3 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APOC3 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APOC3 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-APOC3 antibody
Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
345
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11kDa
NCBI Official Full Name
apolipoprotein C-III
NCBI Official Synonym Full Names
apolipoprotein C3
NCBI Official Symbol
APOC3
NCBI Official Synonym Symbols
APOCIII
NCBI Protein Information
apolipoprotein C-III
UniProt Protein Name
Apolipoprotein C-III
Protein Family
UniProt Gene Name
APOC3
UniProt Synonym Gene Names
Apo-CIII; ApoC-III
UniProt Entry Name
APOC3_HUMAN

NCBI Description

This gene encodes a protein component of triglyceride (TG)-rich lipoproteins (TRLs) including very low density lipoproteins (VLDL), high density lipoproteins (HDL) and chylomicrons. The encoded protein plays a role in role in the metabolism of these TRLs through multiple modes. This protein has been shown to promote the secretion of VLDL1, inhibit lipoprotein lipase enzyme activity, and delay catabolism of TRL remnants. Mutations in this gene are associated with low plasma triglyceride levels and reduced risk of ischemic cardiovascular disease, and hyperalphalipoproteinemia, which is characterized by elevated levels of high density lipoprotein (HDL) and HDL cholesterol in human patients. This gene and other related genes comprise an apolipoprotein gene cluster on chromosome 11. [provided by RefSeq, Sep 2017]

Uniprot Description

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity

Biological Process: response to drug; cholesterol metabolic process; response to peptide hormone stimulus; phototransduction, visible light; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; cholesterol efflux; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; negative regulation of lipid metabolic process; retinoid metabolic process; inflammatory response; triacylglycerol mobilization; negative regulation of fatty acid biosynthetic process; response to nutrient

Disease: Apolipoprotein C-iii Deficiency

Research Articles on APOC3

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Product Notes

The APOC3 apoc3 (Catalog #AAA3012248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOC3 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOC3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the APOC3 apoc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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