Rabbit anti-Human APOBEC3F Polyclonal Antibody | anti-APOBEC3F antibody

APOBEC3F, NT (APOBEC3F, DNA dC->dU-editing enzyme APOBEC-3F, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F) (Azide free) (HRP)

Cat. Num. AAA6274449

• Gene Names: APOBEC3F; A3F; KA6; ARP8; BK150C2.4.MRNA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APOBEC3F; Polyclonal Antibody; NT (APOBEC3F; DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F) (Azide free) (HRP); Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; anti-APOBEC3F antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APOBEC3F.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-APOBEC3F antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 22-48 in the N-terminal region of human APOBEC3F. UniProt Accession #Q8IUX4
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS643632. APOBEC3F (arrow) was detected usingMBS643632.)

Immunohistochemistry (IHC)

(Immmunohistochemistry in formalin-fixed and paraffin-embedded human brain tissue reacted withMBS643632, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS643632 for immunohistochemistry.)

Related Product Information for anti-APOBEC3F antibody

This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.

Product Categories/Family for anti-APOBEC3F antibody

Antibodies; Enzymes; Cytidine Deaminase (APOBEC)

References

1. Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010). 2. Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010). 3. Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010). 4. Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009). 5. Mubiru, J.N., et al. Gene 327(1):89-98(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 161784334
• NCBI GeneID: 200316
• UniProt Accession #: Q8IUX4; Q45F03; Q6ICH3; Q7Z2N2; Q7Z2N5; B0QYD4
• Molecular Weight: 373
• NCBI Official Full Name: DNA dC->
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3F
• NCBI Official Symbol: APOBEC3F
• NCBI Official Synonym Symbols: A3F; KA6; ARP8; BK150C2.4.MRNA
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3F; induced upon T-cell activation; apolipoprotein B mRNA editing enzyme cytidine deaminase; apolipoprotein B editing enzyme catalytic polypeptide-like 3F; apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3F
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3F
• UniProt Synonym Gene Names: A3F
• UniProt Entry Name: ABC3F_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

APOBEC3F: Host cellular restriction factor that may have antiviral activities against exogenous and endogenous viruses, as well as retrotransposons. After being packaged into HIV-1 virions, blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; ribonucleoprotein complex

Molecular Function: protein binding; zinc ion binding; RNA binding; cytidine deaminase activity

Biological Process: negative regulation of retroviral genome replication; base conversion or substitution editing; negative regulation of viral genome replication; innate immune response; cytidine deamination; positive regulation of defense response to virus by host; negative regulation of viral reproduction; defense response to virus