Western Blot (WB)
(Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse PIERCE1 Polyclonal Antibody | anti-C9orf116 antibody
PIERCE1 Antibody
Gene Names
C9orf116; PIERCE1; RbEST47
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
PIERCE1 Antibody is affinity chromatography purified via peptide column.
Synonyms
PIERCE1; Polyclonal Antibody; PIERCE1 Antibody; RbEST47; UPF0691 protein C9orf116; p53-induced expression in RB-null cells protein 1; Pierce1; chromosome 9 open reading frame 116; anti-C9orf116 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PIERCE1 antibody is predicted to not cross-react with other UPF0691 family members.
Purity/Purification
PIERCE1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
136
Applicable Applications for anti-C9orf116 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL.
Conjugate
Unconjugated
Immunogen
PIERCE1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human PIERCE1.
Buffer
PIERCE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
PIERCE1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of PIERCE1 in A20 cell lysate with PIERCE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunocytochemistry (IHC)
(Immunocytochemistry of PIERCE1 in A20 cells with PIERCE1 antibody at 2.5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of PIERCE1 in A20 cells with PIERCE1 antibody at 2.5 μg/mL.)
Related Product Information for anti-C9orf116 antibody
PIERCE1 Antibody: PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation. PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer. PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene. It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,318 Da
NCBI Official Full Name
UPF0691 protein C9orf116 isoform 1
NCBI Official Synonym Full Names
chromosome 9 open reading frame 116
NCBI Official Symbol
C9orf116
NCBI Official Synonym Symbols
PIERCE1; RbEST47
NCBI Protein Information
UPF0691 protein C9orf116; pierce 1; p53-induced expression in RB-null cells protein 1; p53-induced expression 1 in Rb−/− cells
UniProt Protein Name
UPF0691 protein C9orf116
Protein Family
UniProt Gene Name
C9orf116
UniProt Synonym Gene Names
Pierce1
UniProt Entry Name
CI116_HUMAN
Uniprot Description
1700007K13Rik: Belongs to the UPF0691 family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 9q34.3
Similar Products
Product Notes
The C9orf116 c9orf116 (Catalog #AAA150462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIERCE1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PIERCE1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the C9orf116 c9orf116 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIERCE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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