Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells and COS-7cells, using APOBEC3D/F antibody.)
Rabbit anti-Human APOBEC3D/F Polyclonal Antibody | anti-APOBEC3D/F antibody
APOBEC3D/F Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
APOBEC3D/F; Polyclonal Antibody; APOBEC3D/F Antibody; Probable DNA dC->dU-editing enzyme APOBEC-3D; EC 3.5.4.-; APOBEC3D; anti-APOBEC3D/F antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total APOBEC3D/F protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
386
Applicable Applications for anti-APOBEC3D/F antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry: 1:50~1:100
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human APOBEC3D/F.
Target Name
APOBEC3D/F
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells and COS-7cells, using APOBEC3D/F antibody.)
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells and COS-7cells, using APOBEC3D/F antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using APOBEC3D/F antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human placenta tissue using APOBEC3D/F antibody.)
Related Product Information for anti-APOBEC3D/F antibody
DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. May inhibit the mobility of LTR and non-LTR retrotransposons.Dunham I., Nature 402:489-495(1999).Jarmuz A., Genomics 79:285-296(2002).Wedekind J.E., Trends Genet. 19:207-216(2003).
Product Categories/Family for anti-APOBEC3D/F antibody
NCBI and Uniprot Product Information
Uniprot Description
APOBEC3D: Probable DNA cytidine deaminase involved in foreign DNA clearance. May provide cellular innate resistance to a specific panel of genetic invaders including endogenous retroelements and a subset of viruses. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: Hydrolase; EC 3.5.4.-
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: cytoplasm
Molecular Function: zinc ion binding; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
Biological Process: negative regulation of retroviral genome replication; innate immune response; defense response to virus
Similar Products
Product Notes
The APOBEC3D/F apobec3d (Catalog #AAA9403570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOBEC3D/F Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC3D/F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500~1:3000 Immunohistochemistry: 1:50~1:100. Researchers should empirically determine the suitability of the APOBEC3D/F apobec3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC3D/F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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