Western Blot (WB)
(Western Blot (WB) analysis of APOBEC3D/APOBEC3F polyclonal antibody at 1:500 dilutionLane1:H1792 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug))
Rabbit APOBEC3D/APOBEC3F Polyclonal Antibody | anti-APOBEC3D antibody
APOBEC3D/APOBEC3F Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
APOBEC3D/APOBEC3F; Polyclonal Antibody; APOBEC3D/APOBEC3F Polyclonal Antibody; DNA dC->dU-editing enzyme APOBEC-3D; A3D; APOBEC3D; DNA dC->dU-editing enzyme APOBEC-3F; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F; A3F; APOBEC3F; anti-APOBEC3D antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
APOBEC3D/APOBEC3F polyclonal antibody detects endogenous levels of APOBEC3D/APOBEC3F protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
386
Applicable Applications for anti-APOBEC3D antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 220-270 of Human APOBEC3D.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of APOBEC3D/APOBEC3F polyclonal antibody at 1:500 dilutionLane1:H1792 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of APOBEC3D/APOBEC3F polyclonal antibody at 1:500 dilutionLane1:H1792 whole cell lysate (40ug) Lane2:SK-OVCAR3 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug))
Related Product Information for anti-APOBEC3D antibody
DNA dC->dU-editing enzyme APOBEC-3D/3F, DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
~ 45kDa
NCBI Official Full Name
DNA dC->
UniProt Protein Name
DNA dC->dU-editing enzyme APOBEC-3D
Protein Family
UniProt Gene Name
APOBEC3D
UniProt Synonym Gene Names
A3D
Uniprot Description
APOBEC3D: Probable DNA cytidine deaminase involved in foreign DNA clearance. May provide cellular innate resistance to a specific panel of genetic invaders including endogenous retroelements and a subset of viruses. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: EC 3.5.4.-; Hydrolase
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: cytoplasm
Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines; zinc ion binding
Biological Process: defense response to virus; innate immune response; negative regulation of retroviral genome replication
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Product Notes
The APOBEC3D apobec3d (Catalog #AAA3007609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOBEC3D/APOBEC3F Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC3D/APOBEC3F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the APOBEC3D apobec3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC3D/APOBEC3F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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