Rabbit anti-Human API5 Polyclonal Antibody | anti-API5 antibody

API5, CT (API5, Apoptosis inhibitor 5, Antiapoptosis clone 11 protein, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, Protein XAGL) (FITC)

Cat. Num. AAA6274338

• Gene Names: API5; AAC11; AAC-11
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: API5; Polyclonal Antibody; CT (API5; Apoptosis inhibitor 5; Antiapoptosis clone 11 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; Protein XAGL) (FITC); Protein XAGL; anti-API5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-API5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 481-510 amino acids selected from the C-terminal region of human API5.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(API5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the API5 antibody detected the API5 protein (arrow).)

Related Product Information for anti-API5 antibody

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.

Product Categories/Family for anti-API5 antibody

Antibodies; Apoptosis

References

Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010): Ren, K., et al. Pathol. Oncol. Res. 16(2):229-237(2010). Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009). Rigou, P., et al. EMBO J. 28(11):1576-1588(2009). Wang, Y., et al. J. Biol. Chem. 283(19):13205-13215(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5729730
• NCBI GeneID: 8539
• NCBI Accession #: NP_006586.1
• NCBI GenBank Nucleotide #: NM_006595.3
• UniProt Accession #: Q9BZZ5; O15441; Q9Y4J7; B4DGR0; B4DRJ2; D3DR21
• Molecular Weight: 50,740 Da
• NCBI Official Full Name: apoptosis inhibitor 5 isoform b
• NCBI Official Synonym Full Names: apoptosis inhibitor 5
• NCBI Official Symbol: API5
• NCBI Official Synonym Symbols: AAC11; AAC-11
• NCBI Protein Information: apoptosis inhibitor 5; FIF; antiapoptosis clone 11 protein; migration-inducing protein MIG8; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2
• UniProt Protein Name: Apoptosis inhibitor 5
• Protein Family: Apoptosis inhibitor
• UniProt Gene Name: API5
• UniProt Synonym Gene Names: API-5; AAC-11; FIF
• UniProt Entry Name: API5_HUMAN

NCBI Description

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

API5: Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Belongs to the API5 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; RNA-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nucleoplasm; spliceosome; membrane; cytoplasm; nucleus

Molecular Function: protein binding; fibroblast growth factor binding

Biological Process: apoptosis; negative regulation of apoptosis