Rabbit APG5 Polyclonal Antibody | anti-ATG5 antibody

APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5) (HRP)

Cat. Num. AAA620635

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat, Xenopus0
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: APG5; Polyclonal Antibody; APG5 (APG5 Autophagy 5-like; APG5-like; APG5L; Apoptosis-specific Protein; ASP; ATG5 Autophagy-related 5 Homolog; Autophagy Protein 5; hAPG5; Homolog of S Cerevisiae Autophagy 5) (HRP); Anti -APG5 (APG5 Autophagy 5-like; anti-ATG5 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat, Xenopus0
• Clonality: Polyclonal
• Specificity: Selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing aa1-79. The calpain cleaved form of ATG5 is missing aa195-275. Cellular Localization
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, 30% glycerol. Labeled with HRP.

• Applicable Applications for anti-ATG5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500; Immunohistochemistry: 2.5ug/ml; Optimal dilutions to be determined by researcher.
• Immunogen: A synthetic peptide made to an N-terminal region (aa1-50) of the human ATG5 protein.
• Positive Control: Mouse wild-type ES cell lysate.
• Preparation and Storage: Store at 2-8 degree C. Do Not Freeze! Aliquots are stable for at least 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Related Product Information for anti-ATG5 antibody

Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Product Categories/Family for anti-ATG5 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 4757798
• NCBI GeneID: 9474
• NCBI Accession #: NP_004840.1
• NCBI GenBank Nucleotide #: NM_004849.2
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7; Q7Z3H3
• Molecular Weight: 32,447 Da
• NCBI Official Full Name: autophagy protein 5
• NCBI Official Synonym Full Names: ATG5 autophagy related 5 homolog (S. cerevisiae)
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; OTTHUMP00000017823; OTTHUMP00000017824; OTTHUMP00000017825; apoptosis specific protein; apoptosis-specific protein
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Apoptosis; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation