IRF1 recombinant protein

IRF1, 1-114aa, Human, His tag, E Coli

Cat. Num. AAA203098

• Gene Names: IRF1; MAR; IRF-1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: IRF1; 1-114aa; Human; His tag; E Coli; Interferon regulatory factor-1; MAR; Interferon regulatory factor 1; Interferon regulatory factor-1 Interferon regulatory factor 1; IRF 1; IRF-1; MAR1.; IRF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: In 20mM Tris 8.0, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPITRMRMRP WLEMQINSNQ IPGLIWINKE EMIFQIPWKH AAKHGWDINK DACLFRSWAI HTGRYKAGEK EPDPKTWKAN FRCAMNSLPD IEEVKDQSRN KGSSAVRVYR MLPP
• Sequence Length: 325

• Applicable Applications for IRF1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IRF1 recombinant protein

IRF1, Interferon regulatory factor 1, is a member of the interferon regulatory transcription factor (IRF) family which regulates gene expression critical to immune response, hematopoiesis and proliferation. IRF-1 is a transcriptional activator for IFN-alpha, IFN-beta, and IFN-gamma stimulated genes. IRF1 is also a tumor suppressor transcription factor inducing apoptosis of tumorigenic cell lines. Recombinant human IRF1 was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for IRF1 recombinant protein

Hormones & Cytokines

References

Hochhaus A., et al. (1997) Leukemia. 11 (7):933-9.; Liu J., et al. (2005) J Biol Chem. 280 (26):24347-55;

NCBI and Uniprot Product Information

• NCBI GI #: 4504721
• NCBI GeneID: 3659
• NCBI Accession #: NP_002189
• NCBI GenBank Nucleotide #: NM_002198.2
• UniProt Accession #: P10914; Q96GG7
• Molecular Weight: 15 kDa (134 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: interferon regulatory factor 1 isoform 1
• NCBI Official Synonym Full Names: interferon regulatory factor 1
• NCBI Official Symbol: IRF1
• NCBI Official Synonym Symbols: MAR; IRF-1
• NCBI Protein Information: interferon regulatory factor 1
• UniProt Protein Name: Interferon regulatory factor 1
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF1
• UniProt Synonym Gene Names: IRF-1

NCBI Description

The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer. [provided by RefSeq, Aug 2017]

Uniprot Description

Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity include: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58/RIG-I, TNFSF10/TRAIL, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells.