BMPRIB Active Protein | BMPR1B active protein

BMPRIB Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122453

• Gene Names: Bmpr1b; Alk6; SKR6; ALK-6; Acvrlk6; BMPR-1B; BMPR-IB; CFK-43a; AI385617; AV355320
• Purity: >95% as determined by SDS-PAGE
• Synonyms: BMPRIB; BMPRIB Protein; Mouse; Recombinant (hFc Tag); Mouse ALK-6/BMPR1B Protein (Fc Tag); Acvrlk6 Protein; AI385617 Protein; ALK-6 Protein; Alk6 Protein; AV355320 Protein; BMPR-1B Protein; BMPR-IB Protein; CFK-43a Protein; SKR6 Protein; bone morphogenetic protein receptor; type IB; BMPR1B active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys14-Lys126

• Species: Mouse
• Activity: Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells. The ED50 for this effect is 1.5-7.5ug/mL.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Lys 14
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the mouse Bmpr1b (NP_031586.1)(Lys14-Lys126) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 1.5-7.5 ug/mL.)

Related Product Information for BMPR1B active protein

Background: BMPR1B(bone morphogenetic protein receptor, type IB), also known as ALK6, is a member of the bone morphogenetic protein (BMP) receptor family. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. BMPR1B is the major transducer of signals in precartilaginous condensations as demonstrated in experiments using constitutively active BMPR1B receptors. BMPR1B is a more effective transducer of GDF5 than BMPR1A. Unlike BMPR1A null mice, which die at an early embryonic stage, BMPR1B null mice are viable.

References

Ide H, et al. (1998) Assignment of the BMPR1A and BMPR1B genes to human chromosome 10q22.3 and 4q23--q24 byin situ hybridization and radiation hybrid map ping. Cytogenet. Cell Genet. 81(3-4): 285-6.Mishina Y, et al. (2004) Bone morphogenetic protein type IA receptor signaling regulates postnatal osteoblast function and bone remodeling. J Biol Chem. 279(26): 27560-6.Yoon BS, et al. (2005) Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo. Proc Natl Acad Sci. 102(14): 5062-7.

NCBI and Uniprot Product Information

• NCBI GI #: 6680802
• NCBI GeneID: 12167
• NCBI Accession #: NP_031586.1
• NCBI GenBank Nucleotide #: NM_007560.3
• UniProt Accession #: P36898; Q3TRF2
• Molecular Weight: 56,944 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1B
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type 1B
• NCBI Official Symbol: Bmpr1b
• NCBI Official Synonym Symbols: Alk6; SKR6; ALK-6; Acvrlk6; BMPR-1B; BMPR-IB; CFK-43a; AI385617; AV355320
• NCBI Protein Information: bone morphogenetic protein receptor type-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine-protein kinase receptor R6
• UniProt Protein Name: Bone morphogenetic protein receptor type-1B
• Protein Family: Bone morphogenetic protein receptor
• UniProt Gene Name: Bmpr1b
• UniProt Synonym Gene Names: Acvrlk6; BMP type-1B receptor; BMPR-1B; ALK-6; SKR6
• UniProt Entry Name: BMR1B_MOUSE

NCBI Description

This gene encodes a serine/threonine kinase that functions as a receptor for bone morphogenetic proteins (BMPs). The encoded protein is a type I receptor, and forms a complex of two type II and two type I receptors at the cell membrane. This complex signals downstream to activate SMAD transcriptional regulators. This signaling is important in skeletal and bone development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; EC 2.7.11.30; TKL group; STKR family; Type1 subfamily

Cellular Component: membrane; integral to membrane

Molecular Function: transferase activity; transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: camera-type eye development; positive regulation of bone mineralization; protein amino acid phosphorylation; estrogen biosynthetic process; limb morphogenesis; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; dorsal/ventral pattern formation; eye development; retina development in camera-type eye; cartilage development; transforming growth factor beta receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; chondrocyte differentiation; ovarian cumulus expansion; cell differentiation; positive regulation of cell differentiation; phosphorylation; skeletal development; cartilage condensation; retinal ganglion cell axon guidance