Rabbit anti-Human APG10L Polyclonal Antibody | anti-APG10L antibody

APG10L, CT (S116) (ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10) (PE)

Cat. Num. AAA6274224

• Gene Names: ATG10; APG10; APG10L; pp12616
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APG10L; Polyclonal Antibody; CT (S116) (ATG10; Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10) (PE); ATG10; Autophagy-related protein 10; anti-APG10L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APG10L antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: APG10L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-121 amino acids from the C-terminal region of human APG10L.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)

Western Blot (WB)

(Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.)

Immunohistochemistry (IHC)

(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-APG10L antibody

Antibodies; Autophagy

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007). Shao, Y., et al. Autophagy 3(1):10-16(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 196162717
• NCBI GeneID: 83734
• NCBI Accession #: NP_001124500.1
• NCBI GenBank Nucleotide #: NM_001131028.1
• UniProt Accession #: Q9H0Y0; Q6PIX1; Q9H842; B2RE09
• Molecular Weight: 14,217 Da
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG10
• NCBI Official Synonym Full Names: autophagy related 10
• NCBI Official Symbol: ATG10
• NCBI Official Synonym Symbols: APG10; APG10L; pp12616
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG10; ATG10 autophagy related 10 homolog; autophagy-related protein 10
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG10
• UniProt Gene Name: ATG10
• UniProt Synonym Gene Names: APG10L; APG10-like
• UniProt Entry Name: ATG10_HUMAN

NCBI Description

Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: cytoplasm; intracellular

Molecular Function: APG12 conjugating enzyme activity; ligase activity

Biological Process: protein transport; protein modification by small protein conjugation; protein amino acid lipidation; positive regulation of protein modification process; autophagy