Pepsinogen A (PGA) Recombinant Protein | PGA recombinant protein

Recombinant Pepsinogen A (PGA)

Cat. Num. AAA2009721

• Gene Names: Pga5; Pepf
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Pepsinogen A (PGA); Recombinant Pepsinogen A (PGA); PGA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LVKIP LMKIKSMREN LRESHMLKDY LEKYPRSRAH VLLEQRRNPS VTYEPMRNYL DLVYIGTISI GTPPQEFKVV LDTGSSDLWV PSIYCSSPAC AHHKVFNPLQ SSTFLVSGRP VNVAYGSGEM SGFLAYDTVK IGDLTVVAQA FGLSLEEPGR FMEHAVFDGI LGLGYPNLGL QGVTPVFDNL WIQGLIPQNL FAFYLSSKDE KGSVLMLGGV DPSYYHGELH WVPVSKPSYW QLAVDSISMN GEIIACDGGC QGIMDTGTSL VTGPRSSILN IQNLIGAKAS GDGEYFLKCD TINTLPDIVF TIDSVTYPVP ASAYIRKDHS HNCRSNFEES TDDPSDPELW VLGDVFLRLY FTVFDRANNR IGLASAA
• Sequence Length: 387

• Applicable Applications for PGA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: Accurate protein sizing on SDS-polyacrylamide gells and Western Blots
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Leu16~Ala387 (Accession # Q9JJX2) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Predicted Isoelectric Point: 5.6
• Storage BUffer: 62.5mM Tris-H3PO4 (pH 7.5 at 25degC), 1mM EDTA, 2% (w/v) SDS, 100mM DTT, 1mM NaN3, 0.01% (w/v) bromo-phenol blue and 33% (v/v) glycerol.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 11120702
• NCBI GeneID: 60372
• NCBI Accession #: NP_068521.1
• NCBI GenBank Nucleotide #: NM_021753.1
• UniProt Accession #: Q9JJX2
• Molecular Weight: 42.6kDa
• NCBI Official Full Name: pepsinogen 5, group I
• NCBI Official Synonym Full Names: pepsinogen 5, group I
• NCBI Official Symbol: Pga5
• NCBI Official Synonym Symbols: Pepf
• NCBI Protein Information: pepsinogen 5, group I; pepsinogen F protein; pepsinogen 5, group I (pepsinogen A)
• UniProt Protein Name: Pepsinogen 5, group I (Pepsinogen A)
• Protein Family: Pepsin
• UniProt Gene Name: LOC100910889
• UniProt Synonym Gene Names: Pga5
• UniProt Entry Name: Q9JJX2_RAT

NCBI Description

neontal zymogen of pepsins, the major proteolytic enzymes in gastric juices [RGD, Feb 2006]

Uniprot Description

Sequence similarities: Belongs to the peptidase A1 family. RuleBase RU000454