Rabbit anti-Human APCS Polyclonal Antibody | anti-APCS antibody

APCS, CT (APCS, PTX2, Serum amyloid P-component, 9.5S alpha-1-glycoprotein, Serum amyloid P-component(1-203)) (PE)

Cat. Num. AAA6274213

• Gene Names: APCS; SAP; PTX2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APCS; Polyclonal Antibody; CT (APCS; PTX2; Serum amyloid P-component; 9.5S alpha-1-glycoprotein; Serum amyloid P-component(1-203)) (PE); Serum amyloid P-component(1-203); anti-APCS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APCS.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APCS antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 151-178 in the C-terminal region of human APCS, UniProt Accession #NP_001630.1; P02743; Q13449.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS647035. This demonstrates that MBS647035 detected the APCS protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS647035 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-APCS antibody

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Product Categories/Family for anti-APCS antibody

Antibodies; Amyloid

References

1. Song, Z., et al. Atherosclerosis 211(1):90-95(2010). 2. Davila, S., et al. Genes Immun. 11(3):232-238(2010). 3. McGeachie, M., et al. Circulation 120(24):2448-2454(2009). 4. Lu, J., et al. Nature 456(7224):989-992(2008). 5. Verwey, N.A., et al. Dement Geriatr Cogn Disord 26(6):522-527(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4502133
• NCBI GeneID: 325
• NCBI Accession #: NP_001630.1
• NCBI GenBank Nucleotide #: NM_001639.3
• UniProt Accession #: P02743; Q13449
• Molecular Weight: 25,387 Da
• NCBI Official Full Name: serum amyloid P-component
• NCBI Official Synonym Full Names: amyloid P component, serum
• NCBI Official Symbol: APCS
• NCBI Official Synonym Symbols: SAP; PTX2
• NCBI Protein Information: serum amyloid P-component; pentaxin-related; 9.5S alpha-1-glycoprotein
• UniProt Protein Name: Serum amyloid P-component
• UniProt Gene Name: APCS
• UniProt Synonym Gene Names: PTX2; SAP
• UniProt Entry Name: SAMP_HUMAN

NCBI Description

The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]

Uniprot Description

APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.

Protein type: Secreted, signal peptide; Secreted; Chaperone

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus

Molecular Function: complement component C1q binding; unfolded protein binding; virion binding; calcium ion binding; carbohydrate binding

Biological Process: protein folding; negative regulation of virion penetration into host cell; acute-phase response; negative regulation of acute inflammatory response; innate immune response; chaperone-mediated protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction