Rabbit Apaf 1 Apoptosis Activating Protease Fact Polyclonal Antibody | anti-APAF1 antibody

Apaf 1 (NT) Apoptosis Activating Protease Fact

Cat. Num. AAA395960

• Gene Names: APAF1; CED4; APAF-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: Apaf 1 Apoptosis Activating Protease Fact; Polyclonal Antibody; Apaf 1 (NT) Apoptosis Activating Protease Fact; anti-APAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative
• Sequence Length: 1194

• Applicable Applications for anti-APAF1 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to amino acids 12 to 28 of the human Apaf-1 protein. The immunogen sequence is identical in human and mouse
• Positive Control: Whole HeLa cell lysate
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-APAF1 antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues (1).

Product Categories/Family for anti-APAF1 antibody

Polyclonal Antibody; Caspases

NCBI and Uniprot Product Information

• NCBI GI #: 4502123
• NCBI GeneID: 317
• NCBI Accession #: NP_001151.1
• NCBI GenBank Nucleotide #: NM_001160.2
• UniProt Accession #: O14727; O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8
• Molecular Weight: 130
• NCBI Official Full Name: apoptotic protease-activating factor 1 isoform b
• NCBI Official Synonym Full Names: apoptotic peptidase activating factor 1
• NCBI Official Symbol: APAF1
• NCBI Official Synonym Symbols: CED4; APAF-1
• NCBI Protein Information: apoptotic protease-activating factor 1
• UniProt Protein Name: Apoptotic protease-activating factor 1
• Protein Family: Apoptotic protease-activating factor
• UniProt Gene Name: APAF1
• UniProt Synonym Gene Names: KIAA0413; APAF-1
• UniProt Entry Name: APAF_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Ref.4 Ref.5

Subunit structure: Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Ref.4 Ref.12 Ref.14 Ref.16

Subcellular location: Cytoplasm Ref.5.

Tissue specificity: Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Induction: By E2F and p53/TP53 in apoptotic neurons. Ref.13

Domain: The CARD domain mediates interaction with APIP.The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization

By similarity.

Miscellaneous: Physiological concentrations of calcium ions negatively affect the assembly of apoptosome by inhibiting nucleotide exchange in the monomeric form.

Sequence similarities: Contains 1 CARD domain.Contains 1 NB-ARC domain.Contains 15 WD repeats.

Sequence caution: The sequence AAK28401.1 differs from that shown. Reason: Frameshift at position 108. The sequence BAA24843.2 differs from that shown. Reason: Erroneous initiation.