Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit | PPP1CA elisa kit

Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit

Cat. Num. AAA9428821

• Gene Names: PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
• Reactivity: Human
• Synonyms: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit ELISA Kit; PP-1A; PPP1CA; PPP1A; 3.1.3.16; PPP1CA elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Syntenin-1. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.312-20 ng/ml
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for PPP1CA elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of humanSyntenin-1concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syntenin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forSyntenin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Syntenin- 1, biotin- conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Syntenin- 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 56790945
• NCBI GeneID: 5499
• NCBI Accession #: NP_001008709.1
• NCBI GenBank Nucleotide #: NM_001008709.1
• UniProt Accession #: P62136; P08129; P20653; P22802; Q07161; A6NNR3; B2R908
• Molecular Weight: 32,595 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
• NCBI Official Synonym Full Names: protein phosphatase 1 catalytic subunit alpha
• NCBI Official Symbol: PPP1CA
• NCBI Official Synonym Symbols: PP1A; PP-1A; PPP1A; PP1alpha
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP1CA
• UniProt Synonym Gene Names: PPP1A; PP-1A

NCBI Description

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PPP1CA: is a catalytic subunit of protein phosphatase 1 (PP1), aubiquitous cytosolic serine-threonine phosphatase. Composed of a catalytic subunit (PPP1CA, PPP1CB or PPP1CC) which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting (or regulatory) subunits: PPP1R12A and PPP1R12B mediate binding to myosin; PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen; and PPP1R15A and PPP1R15B mediate binding to EIF2S1. PP1 is essential for cell division, plays critical roles in many cellular processes including glycogen metabolism, muscle contractility and protein synthesis, and is involved in the regulation of ionic conductances and long-term synaptic plasticity. Inhibits the synthesis of proteins involved in synaptic plasticity. Binds to the transcription factor cyclic AMP-dependent response element binding (CREB) and dephosphorylates it. May regulate chromatin condensation through regulation of histone H3 phosphorylation. Differentially expressed in gastric cancer. May participate in the neurodegenerative progress in Alzheimer's disease. Down-regulated in lung squamous cell carcinoma.

Protein type: EC 3.1.3.16; Nucleolus; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; nuclear chromosome, telomeric region; nucleoplasm; nucleus; plasma membrane

Molecular Function: phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein binding

Biological Process: circadian regulation of gene expression; dephosphorylation; entrainment of circadian clock by photoperiod; negative regulation of protein binding; protein amino acid dephosphorylation; regulation of circadian rhythm