Immunofluorescence (IF)
(Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)
Rabbit anti-Human Annexin I Polyclonal Antibody | anti-ANXA1 antibody
Annexin I Polyclonal Antibody
IF: 1:50-1:200
IHC-P: 1:200-1:500
ELISA: 1:10000-1:20000
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Immunofluorescence (IF)
(Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.1. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.2. Immunofluorescence analysis of human-breast tissue. 1, Annexin I Polyclonal Antibody (red) was diluted at 1:200 (4 degree C,overnight). 2, Cy3 Labeled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Annexin I Polyclonal antibody was diluted at 1:200 (4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.3. Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1, Annexin I Polyclonal antibody was diluted at 1:200 (4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.5. Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]
Uniprot Description
ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Calcium-binding; Lipid-binding
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: extracellular space; protein complex; focal adhesion; cell surface; mast cell granule; basolateral plasma membrane; extracellular region; cornified envelope; cilium; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma
Molecular Function: protein binding, bridging; protein homodimerization activity; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; calcium ion binding; calcium-dependent protein binding; helicase activity; protein binding; single-stranded RNA binding; double-stranded DNA-dependent ATPase activity; phospholipid binding; structural molecule activity; single-stranded DNA binding; receptor binding
Biological Process: prostate gland development; response to drug; response to peptide hormone stimulus; positive regulation of neutrophil apoptosis; gliogenesis; positive regulation of vesicle fusion; alpha-beta T cell differentiation; arachidonic acid secretion; neutrophil homeostasis; signal transduction; endocrine pancreas development; response to estradiol stimulus; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; cell motility; inflammatory response; response to X-ray; positive regulation of prostaglandin biosynthetic process; negative regulation of apoptosis
Research Articles on ANXA1
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Product Notes
The ANXA1 anxa1 (Catalog #AAA9700776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Annexin I Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IF: 1:50-1:200 IHC-P: 1:200-1:500 ELISA: 1:10000-1:20000. Researchers should empirically determine the suitability of the ANXA1 anxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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