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Western Blot (WB) (Western Blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution. Positive control: Lane1: Siha Lane2: HelaLane3: Rat spleen tissue Lane4: Rat liver tissueLane5: Mouse lung tissue)

Rabbit Annexin A3 Polyclonal Antibody | anti-ANXA3 antibody

Annexin A3 Polyclonal Antibody

Gene Names
ANXA3; ANX3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified.
Synonyms
Annexin A3; Polyclonal Antibody; Annexin A3 Polyclonal Antibody; PAP-III; ANXA3; ANX3; 2-cyclic phosphate 2-phosphohydrolase; 35-alpha calcimedin; Annexin III; Annexin-3; ANXA 3; Anxa3; Calcimedin 35 alpha; Inositol 1 2 cyclic phosphate 2 phosphohydrolase; Inositol 1; Lipocortin III; OTTHUMP00000160701; OTTHUMP00000219237; OTTHUMP00000219238; PAP III; Placental anticoagulant protein III; anti-ANXA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Annexin A3 polyclonal antibody detects endogenous levels of Annexin A3 protein.
Purity/Purification
Protein affinity purified.
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
323
Applicable Applications for anti-ANXA3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein with Human Annexin A3 aa 1-350.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution. Positive control: Lane1: Siha Lane2: HelaLane3: Rat spleen tissue Lane4: Rat liver tissueLane5: Mouse lung tissue)

Western Blot (WB) (Western Blot analysis of Annexin A3 on different lysates using anti-Annexin A3 antibody at 1/1,000 dilution. Positive control: Lane1: Siha Lane2: HelaLane3: Rat spleen tissue Lane4: Rat liver tissueLane5: Mouse lung tissue)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Annexin A3 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-ANXA3 antibody
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis, exocytosis and cellular adhesion. Annexin III, also known as ANXA3, Annexin A3, Lipocortin III, PAP-III (placental anticoagulant protein III), 35-alpha calcimedin or ANX3, belongs to the annexin family and contains four Annexin repeats. Annexin III exhibits a variety of functions, including anti-coagulent properties, an inhibitory role towards phospholipase A2 (PLA2) and an enzymatic function, cleaving the cyclic bond of inositol 1, 2-cyclic phosphate to produce inositol 1-phosphate.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
306
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36kDa
NCBI Official Full Name
annexin A3
NCBI Official Synonym Full Names
annexin A3
NCBI Official Symbol
ANXA3
NCBI Official Synonym Symbols
ANX3
NCBI Protein Information
annexin A3
UniProt Protein Name
Annexin A3
Protein Family
UniProt Gene Name
ANXA3
UniProt Synonym Gene Names
ANX3; PAP-III
UniProt Entry Name
ANXA3_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA3: a calcium/phospholipid-binding protein and inhibitor of phospholipase A2, Possesses anti-coagulant properties. Cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 4q21.21

Cellular Component: specific granule; phagocytic vesicle membrane; membrane; cytoplasm; plasma membrane

Molecular Function: calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; calcium ion binding; calcium-dependent protein binding

Biological Process: positive regulation of angiogenesis; negative regulation of catalytic activity; neutrophil degranulation; defense response to bacterium; positive regulation of transcription factor activity; phagocytosis

Research Articles on ANXA3

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Product Notes

The ANXA3 anxa3 (Catalog #AAA3012230) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Annexin A3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2,000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the ANXA3 anxa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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