Rabbit anti-Human, Mouse ANGPT2 Polyclonal Antibody | anti-ANGPT2 antibody

ANGPT2, CT (ANGPT2, Angiopoietin-2)

Cat. Num. AAA646909

• Gene Names: ANGPT2; ANG2; AGPT2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by ammonium sulfate precipitation.
• Synonyms: ANGPT2; Polyclonal Antibody; CT (ANGPT2; Angiopoietin-2); Anti -ANGPT2; anti-ANGPT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANGPT2. Species crossreactivity: mouse
• Purity/Purification: Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
• Concentration: 1.88mg/ml (varies by lot)

• Applicable Applications for anti-ANGPT2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Western Blot: 1:2000; Immunohistochemistry (FFPE): 1:50-1:100; Flow Cytometry: 1:25
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 404-432 from the C-terminal region of human ANGPT2. Cellular Localization: secreted
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(ANGPT2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the ANGPT2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC)

(ANGPT2 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(ANGPT2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ANGPT2 antibody detected the ANGPT2 protein (arrow).)

Western Blot (WB)

(ANGPT2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the ANGPT2 antibody detected the ANGPT2 protein (arrow).)

Related Product Information for anti-ANGPT2 antibody

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.

Product Categories/Family for anti-ANGPT2 antibody

Antibodies; Abs to Angiopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 169646754
• NCBI GeneID: 285
• NCBI Accession #: NP_001112360.1
• NCBI GenBank Nucleotide #: NM_001118888.1
• UniProt Accession #: O15123; Q9NRR7; Q9P2Y7; A0AV38; A8K205; B7ZLM7
• NCBI Official Full Name: angiopoietin-2 isoform c
• NCBI Official Synonym Full Names: angiopoietin 2
• NCBI Official Symbol: ANGPT2
• NCBI Official Synonym Symbols: ANG2; AGPT2
• NCBI Protein Information: angiopoietin-2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a
• UniProt Protein Name: Angiopoietin-2
• Protein Family: Angiopoietin
• UniProt Gene Name: ANGPT2
• UniProt Synonym Gene Names: ANG-2
• UniProt Entry Name: ANGP2_HUMAN

NCBI Description

The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Ref.1 Ref.9 Ref.10 Ref.11

Subunit structure: Interacts with TEK/TIE2, competing for the same binding site as ANGPT1. Ref.1 Ref.8 Ref.9 Ref.11

Subcellular location: Secreted.

Domain: The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.