HOXA1 recombinant protein

HOXA1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121207

• Gene Names: HOXA1; BSAS; HOX1; HOX1F
• Purity: >85% as determined by SDS-PAGE
• Synonyms: HOXA1; HOXA1 Protein; Human; Recombinant (His Tag); Human HOXA1 Protein (His Tag); BSAS Protein; HOX1 Protein; HOX1F Protein; homeobox A1; HOXA1 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 30% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-His335

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human HOXA1 isoform 3 (P49639-1)(Met 1-His 335) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HOXA1 recombinant protein

Background: Homeobox protein Hox-A1 is a transcription factor encoded by the HOXA1 gene. This gene is one of the four types of homeobox genes each of which contains a homeobox DNA sequence that codes for the homeodomain, a region of 60 amino acids responsible for the DNA binding exhibited by these homeobox proteins. These Homeobox genes are essential metazoan genes as they determine the identity of embryonic regions along the anterior-posterior axis. The homeobox protein Hox-A1 may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Early in its development, the vertebrate hindbrain is transiently subdivided into a series of compartments called rhombomeres. Genes have been identified whose expression patterns distinguish these cellular compartments. Two of these genes, Hoxa1 and Hoxa2, are required for proper patterning of the early mouse hindbrain and the associated neural crest. It has been detected HOXA1 expression in a variety of human breast cancer lesions, suggesting that HOXA1 may be required for the establishment of breast cancer cell phenotype.

References

Barrow JR, et al. (2000) Roles of Hoxa1 and Hoxa2 in patterning the early hindbrain of the mouse. Development. 127: 933-44.Hong YS, et al. (1995) Structure and function of the HOXA1 human homeobox gene cDNA. Gene. 159 (2): 209-14.Gavalas A, et al. (2001) Synergy between Hoxa1 and Hoxb1: the relationship between arch patterning and the generation of cranial neural crest. Development. 128: 3017-27.

NCBI and Uniprot Product Information

• NCBI GI #: 5031761
• NCBI GeneID: 3198
• NCBI Accession #: NP_005513.1
• NCBI GenBank Nucleotide #: NM_005522.4
• UniProt Accession #: P49639; O43363; A4D184; B2R8U7
• Molecular Weight: 36,641 Da
• NCBI Official Full Name: homeobox protein Hox-A1 isoform a
• NCBI Official Synonym Full Names: homeobox A1
• NCBI Official Symbol: HOXA1
• NCBI Official Synonym Symbols: BSAS; HOX1; HOX1F
• NCBI Protein Information: homeobox protein Hox-A1; homeobox 1F; homeo box A1; lab-like protein; Hox 1.6-like protein; homeobox protein Hox-1F; HOX A1 homeodomain protein
• UniProt Protein Name: Homeobox protein Hox-A1
• Protein Family: Homeobox protein
• UniProt Gene Name: HOXA1
• UniProt Synonym Gene Names: HOX1F
• UniProt Entry Name: HXA1_HUMAN

NCBI Description

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. The encoded protein may be involved in the placement of hindbrain segments in the proper location along the anterior-posterior axis during development. Two transcript variants encoding two different isoforms have been found for this gene, with only one of the isoforms containing the homeodomain region. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXA1: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments. Defects in HOXA1 are the cause of Athabaskan brainstem dysgenesis syndrome (ABDS); also known as Narvajo brainstem syndrome. This syndrome is characterized by horizontal gaze palsy, sensorineural deafness, central hypoventilation, and developmental delay. Some patients had swallowing dysfunction, vocal cord paralysis, facial paresis, seizures, and cardiac outflow tract anomalies. Defects in HOXA1 are the cause of Bosley-Salih-Alorainy syndrome (BSAS). Affected individuals show horizontal gaze abnormalities, deafness, facial weakness, vascular malformations of the internal carotid arteries and cardiac outflow trac. Some patients manifest mental retardation and autism spectrum disorder. In contrast to individuals with ABSD, central hypoventilation is not observed in individuals with BSAS. Belongs to the Antp homeobox family. Labial subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 7p15.3

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: facial nerve structural organization; anatomical structure morphogenesis; central nervous system neuron differentiation; facial nucleus development; transcription, DNA-dependent; multicellular organismal development; neuromuscular process; outer ear morphogenesis; motor axon guidance; rhombomere 4 development; anterior/posterior pattern formation; sensory perception of sound; optokinetic behavior; artery morphogenesis; embryonic neurocranium morphogenesis; positive regulation of transcription from RNA polymerase II promoter; cognition; rhombomere 5 development; inner ear development; rhombomere 3 development; regulation of behavior; abducens nerve formation

Disease: Athabaskan Brainstem Dysgenesis Syndrome